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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

Research Areas
Cell Cycle Pathways
Protein Family And Group
Infectious Disease
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Catalog Number Size Price
LS-C2771-100 100 µl (1 mg/ml) $335 
BUB1B / BubR1 Antibody - Immunofluorescence of an asynchronous cycling population of human cells (U2OS) with BUBR1 [8G1] antibody. No signal is detected from interphase cells, whereas cells undergoing mitosis accumulate BubR1 at the kinetochores. Image reveals kinetochores at prometaphase.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
BUB1B / BubR1 Antibody - BUBR1 detected in HeLa cell lysate using BUBR1 [8G1] antibody.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
BUB1B / BubR1 Antibody - Immunofluorescence of an asynchronous cycling population of human cells (U2OS) with BUBR1 [8G1] antibody. No signal is detected from interphase cells, whereas cells undergoing mitosis accumulate BubR1 at the kinetochores. Image reveals kinetochores at prometaphase.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
BUB1B / BubR1 Antibody - BUBR1 detected in HeLa cell lysate using BUBR1 [8G1] antibody.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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Monoclonal Mouse anti‑Human BUB1B / BubR1 Antibody (clone 8G1, aa1‑350, IF, WB) LS‑C2771

Monoclonal Mouse anti‑Human BUB1B / BubR1 Antibody (clone 8G1, aa1‑350, IF, WB) LS‑C2771

Antibody:
Mouse Monoclonal to Human BUB1B / BubR1
Application:
IF, WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$335 
LS-C2771-100
100 µl
1 mg/ml
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Mouse Monoclonal to Human BUB1B / BubR1
Application:
IF, WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
BubR1 antibody LS-C2771 is an unconjugated mouse monoclonal antibody to BubR1 (BUB1B) (aa1-350) from human. It is reactive with human and mouse. Validated for IF and WB. Cited in 2 publications.
Target
Human BUB1B / BubR1
Synonyms
BUB1B | Bub1A | BUBR1 | HBUBR1 | Protein SSK1 | SSK1 | BUB1beta | MAD3L
Host
Mouse
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal
Clone
8G1
Conjugations
Unconjugated. Also available conjugated with Biotin, DY650, DY488, DY550.
Purification
Protein G purified
Modifications
Unmodified
Applications
  • Immunofluorescence (1:100)
  • Western blot (1:2000 - 1:5000)
Immunogen
A portion of hBUBR1 tagged at the N-Terminus with pMAL (aa 1-350 of human BUBR1).
Epitope
aa1-350
Specificity
hBUBR1. There should be no cross-reactivity with hBUB1.
Presentation
PBS, 0.1% Sodium Azide
Storage
Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About BUB1B / BubR1
O60566 NM_001211 NP_001202.4

LSBio Ratings

BUB1B / BubR1 Antibody (aa1-350, clone 8G1) for IF/Immunofluorescence, WB/Western LS-C2771 has an LSBio Rating of
Publications (4.1)

Publications (2)

Phosphorylation of Astrin Regulates Its Kinetochore Function. Chung HJ, Park JE, Lee NS, Kim H, Jang CY. The Journal of biological chemistry. 2016 291:17579-92. (WB; Human)

DDA3 and Mdp3 modulate Kif2a recruitment onto the mitotic spindle to control minus-end spindle dynamics. Kwon HJ, Park JE, Song H, Jang CY. Journal of cell science. 2016 129:2719-25. (WB; Human)

Customer Reviews (0)


Requested From: United States
Date Requested: 6/4/2020