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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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BMP7 Antibody (aa315‑431) IHC‑plus™ LS‑B7431
Note: This antibody replaces LS-C104486
BMP7 antibody LS-B7431 is an unconjugated rabbit polyclonal antibody to human BMP7. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B7431-50
50 µg
$425
Description
BMP7 antibody LS-B7431 is an unconjugated rabbit polyclonal antibody to human BMP7. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human BMP7
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (2.5 µg/ml)
  • Western blot (0.1 - 0.2 µg/ml)
  • ELISA (0.5 - 2 µg/ml)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
BMP7 antibody was raised against cHO cell derived recombinant Human BMP-7.
Epitope
aa315-431
Specificity
Human BMP-7
Usage
Immunohistochemistry: LS-B7431 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 2.5 ug/ml is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Human BMP-7 (100 ng/ml), a concentration of 0.4-0.6 ug/ml of this antibody is required. ELISA: To detect hBMP-7 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human BMP-7 (LS-C104294) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hBMP-7. Western Blot: To detect hBMP-7 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBMP-7 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
Presentation
Lyophilized from PBS, pH 7.2
Reconstitution
50 µl Sterile water
Storage
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to 2 weeks at 2°C to 8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
Restrictions
For research use only.
About BMP7
P18075 NM_001719 NP_001710.1

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Applications: IHC, IHC - Paraffin, Western blot
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Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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Images

Immunohistochemistry

Anti-BMP7 antibody IHC of human kidney, tubules. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.
Anti-BMP7 antibody IHC of human kidney, tubules. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.

Western blot

Western Blot (reducing) of BMP7 antibody
Western Blot (reducing) of BMP7 antibody

Western blot

Western Blot (non-reducing) of BMP7 antibody
Western Blot (non-reducing) of BMP7 antibody

Enzyme-Linked Immunosorbent Assay

Sandwich ELISA of BMP7 antibody
Sandwich ELISA of BMP7 antibody

Enzyme-Linked Immunosorbent Assay

Anti-Human BMP-7 Sandwich ELISA
Anti-Human BMP-7 Sandwich ELISA

Immunohistochemistry

Anti-BMP7 antibody IHC of human kidney, tubules. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.
Anti-BMP7 antibody IHC of human kidney, tubules. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.

Western blot

Western Blot (reducing) of BMP7 antibody
Western Blot (reducing) of BMP7 antibody

Western blot

Western Blot (non-reducing) of BMP7 antibody
Western Blot (non-reducing) of BMP7 antibody

Enzyme-Linked Immunosorbent Assay

Sandwich ELISA of BMP7 antibody
Sandwich ELISA of BMP7 antibody

Enzyme-Linked Immunosorbent Assay

Anti-Human BMP-7 Sandwich ELISA
Anti-Human BMP-7 Sandwich ELISA

Immunohistochemistry

Anti-BMP7 antibody IHC of human kidney, tubules. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.
Anti-BMP7 antibody IHC of human kidney, tubules. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.

Western blot

Western Blot (reducing) of BMP7 antibody
Western Blot (reducing) of BMP7 antibody

Western blot

Western Blot (non-reducing) of BMP7 antibody
Western Blot (non-reducing) of BMP7 antibody

Enzyme-Linked Immunosorbent Assay

Sandwich ELISA of BMP7 antibody
Sandwich ELISA of BMP7 antibody

Enzyme-Linked Immunosorbent Assay

Anti-Human BMP-7 Sandwich ELISA
Anti-Human BMP-7 Sandwich ELISA

Immunohistochemistry

Anti-BMP7 antibody IHC of human kidney, tubules. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.
Anti-BMP7 antibody IHC of human kidney, tubules. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.

Western blot

Western Blot (reducing) of BMP7 antibody
Western Blot (reducing) of BMP7 antibody

Western blot

Western Blot (non-reducing) of BMP7 antibody
Western Blot (non-reducing) of BMP7 antibody

Enzyme-Linked Immunosorbent Assay

Sandwich ELISA of BMP7 antibody
Sandwich ELISA of BMP7 antibody

Enzyme-Linked Immunosorbent Assay

Anti-Human BMP-7 Sandwich ELISA
Anti-Human BMP-7 Sandwich ELISA

Requested From: United States
Date Requested: 12/11/2018
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