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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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BMP7 Antibody (Biotin) LS‑C104294
Note: This antibody replaces LS-C43128, LS-C35169
BMP7 antibody LS-C104294 is a biotin-conjugated rabbit polyclonal antibody to human BMP7. Validated for ELISA and WB.
Catalog
Size
Price
LS-C104294-25
25 µg
$285
LS-C104294-50
50 µg
$325
Description
BMP7 antibody LS-C104294 is a biotin-conjugated rabbit polyclonal antibody to human BMP7. Validated for ELISA and WB.
Target
Human BMP7
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Biotin. Also available Unconjugated.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.1 - 0.2 µg/ml)
  • ELISA (0.25 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
BMP7 antibody was raised against cHO cell derived recombinant Human BMP-7/OP-1.
Specificity
Human BMP-7 /OP-1
Usage
ELISA: To detect hBMP-7 by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hBMP-7. To detect hBMP-7 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human BMP-7 (LS-B7431) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hBMP-7. Western Blot: To detect hBMP-7 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBMP-7 is 1.5-3 ng/lane, under either reducing or non-reducing conditions. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
Lyophilized from PBS, pH 7.2
Reconstitution
Sterile PBS containing 0.1% BSA
Storage
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to 2 weeks at 2°C to 8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
Restrictions
For research use only.
About BMP7
P18075 NM_001719 NP_001710.1

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Applications: IHC, IHC - Paraffin, Western blot
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Western blot Image
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Images

Western blot

Western Blot (reducing) of BMP7 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot (reducing) of BMP7 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western Blot (non-reducing) of BMP7 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot (non-reducing) of BMP7 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Enzyme-Linked Immunosorbent Assay

Biotinylated Anti-Human BMP-7 Sandwich ELISA
Biotinylated Anti-Human BMP-7 Sandwich ELISA

Western blot

Western Blot (reducing) of BMP7 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot (reducing) of BMP7 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western Blot (non-reducing) of BMP7 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot (non-reducing) of BMP7 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Enzyme-Linked Immunosorbent Assay

Biotinylated Anti-Human BMP-7 Sandwich ELISA
Biotinylated Anti-Human BMP-7 Sandwich ELISA

Western blot

Western Blot (reducing) of BMP7 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot (reducing) of BMP7 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western Blot (non-reducing) of BMP7 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot (non-reducing) of BMP7 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Enzyme-Linked Immunosorbent Assay

Biotinylated Anti-Human BMP-7 Sandwich ELISA
Biotinylated Anti-Human BMP-7 Sandwich ELISA

Western blot

Western Blot (reducing) of BMP7 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot (reducing) of BMP7 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western Blot (non-reducing) of BMP7 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot (non-reducing) of BMP7 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Enzyme-Linked Immunosorbent Assay

Biotinylated Anti-Human BMP-7 Sandwich ELISA
Biotinylated Anti-Human BMP-7 Sandwich ELISA

Requested From: United States
Date Requested: 12/15/2018
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