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Catalog Number Size Price
LS-C392607-200 200 µl $393 
BID Antibody - Antibody staining BID in human bladder sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH 6). Samples were incubated with primary antibody (1:25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
BID Antibody - Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A549 (human lung adenocarcinoma epithelial cell line) cells labeling Pdx1 with antibody at 1:25 dilution, followed by Dylight 488-conjugated goat anti-mouse IgG secondary antibody at 1:200 dilution (green). Immunofluorescence image showing cytoplasm staining on A549 cell line. Cytoplasmic actin is detected with Dylight 554 Phalloidin (PD18466410) at 1:100 dilution (red). The nuclear counter stain is DAPI (blue).
BID Antibody - All lanes: Anti-BID Antibody at 1:4000 dilution. Lane 1: Jurkat whole cell lysates. Lane 2: A431 whole cell lysates. Lane 3: THP-1 whole cell lysates. Lane 4: 293T/17 whole cell lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 22 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
BID Antibody - Antibody staining BID in human bladder sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH 6). Samples were incubated with primary antibody (1:25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
BID Antibody - Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A549 (human lung adenocarcinoma epithelial cell line) cells labeling Pdx1 with antibody at 1:25 dilution, followed by Dylight 488-conjugated goat anti-mouse IgG secondary antibody at 1:200 dilution (green). Immunofluorescence image showing cytoplasm staining on A549 cell line. Cytoplasmic actin is detected with Dylight 554 Phalloidin (PD18466410) at 1:100 dilution (red). The nuclear counter stain is DAPI (blue).
BID Antibody - All lanes: Anti-BID Antibody at 1:4000 dilution. Lane 1: Jurkat whole cell lysates. Lane 2: A431 whole cell lysates. Lane 3: THP-1 whole cell lysates. Lane 4: 293T/17 whole cell lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 22 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
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Monoclonal Mouse anti‑Human BID Antibody (clone 1519CT649.18.47, IHC, IF, WB) LS‑C392607

Monoclonal Mouse anti‑Human BID Antibody (clone 1519CT649.18.47, IHC, IF, WB) LS‑C392607

Antibody:
BID Mouse anti-Human Monoclonal (1519CT649.18.47) Antibody
Application:
IHC, IHC-P, IF, WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$393
LS-C392607-200
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
BID Mouse anti-Human Monoclonal (1519CT649.18.47) Antibody
Application:
IHC, IHC-P, IF, WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
BID antibody LS-C392607 is an unconjugated mouse monoclonal antibody to human BID. Validated for IF, IHC, Peptide-ELISA and WB.
Target
Human BID
Synonyms
BID | Apoptic death agonist | BID isoform L(2) | BID isoform Si6 | Bh3 | FP497 | p22 BID | BID isoform ES(1b) | Desmocollin type 4 | Human BID coding sequence
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal
Clone
1519CT649.18.47
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:25)
  • Immunofluorescence (1:25)
  • Western blot (1:4000)
  • Peptide Enzyme-Linked Immunosorbent Assay
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About BID
P55957 NM_001196 NP_001187.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

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Requested From: United States
Date Requested: 5/20/2024