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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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BID Antibody LS‑C148235

Catalog Number Size Price
LS-C148235-50 50 µl $335 
Two cores (A and B) from a human ovarian carcinoma formalin-fixed, paraffin-embedded tissue microarray stained for Bid expression using Polyclonal Antibody to Bid at 1:2000. Hematoxylin-Eos in counterstain.
Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue array stained for Bid expression using Polyclonal Antibody to Bid at 1:2000. Hematoxylin-Eosin counterstain. Variable Bid expression is seen between patient samples.
Western blot of Bid in normal mouse tissues using Polyclonal Antibody to Bid at 1:2000. BID = recombinant Bid. Arrowheads indicate the positions of the full-length (uncleaved) ~22 kD Bid and the ~15 kD truncated form of Bid (tBid) typical of the caspase-cleavage. Additional bands representing partial Bid degradation products are indicated by asterisks (*).
Two cores (A and B) from a human ovarian carcinoma formalin-fixed, paraffin-embedded tissue microarray stained for Bid expression using Polyclonal Antibody to Bid at 1:2000. Hematoxylin-Eos in counterstain.
Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue array stained for Bid expression using Polyclonal Antibody to Bid at 1:2000. Hematoxylin-Eosin counterstain. Variable Bid expression is seen between patient samples.
Western blot of Bid in normal mouse tissues using Polyclonal Antibody to Bid at 1:2000. BID = recombinant Bid. Arrowheads indicate the positions of the full-length (uncleaved) ~22 kD Bid and the ~15 kD truncated form of Bid (tBid) typical of the caspase-cleavage. Additional bands representing partial Bid degradation products are indicated by asterisks (*).
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BID Antibody LS‑C148235

Antibody:
Rabbit Polyclonal to Mouse BID
Application:
IHC, IHC-P, IHC-Fr, WB, IP
Reactivity:
Mouse, Human, Rat, Dog, Gerbil
Format:
Unconjugated, Unmodified
Price
Catalog Number
$335 
LS-C148235-50
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Mouse BID
Application:
IHC, IHC-P, IHC-Fr, WB, IP
Reactivity:
Mouse, Human, Rat, Dog, Gerbil
Format:
Unconjugated, Unmodified

Specifications

Description
BID antibody LS-C148235 is an unconjugated rabbit polyclonal antibody to BID from human, mouse, rat and other species. Validated for IHC, IP and WB.
Target
Mouse BID
Host
Rabbit
Reactivity
Mouse, Human, Rat, Dog, Gerbil (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Antiserum
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:1000 - 1:5000)
  • IHC - Frozen
  • Western blot (1:1000 - 1:2000)
  • Immunoprecipitation (1:50 - 1:200)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Full length recombinant mouse Bid protein.
Specificity
Full-length Bid is known to undergo cleavage/truncation (reviewed in Yin, 2006). Bid was initially found to be cleaved and activated by caspase-8 following death receptor activation. The term "Bid" was first used to described the caspase-8 cleaved/truncated C-terminal Bid. Bid can be also cleaved by other proteases such as Granzyme B, calpains and cathepsin. The C-terminal portion is considered to be the active Bid moiety. This active form can translocate from the cytosol to the mitochondria. Therefore, the appearance of Bid in the mitochondria is considered to be an indication of active Bid. However, it should also be noted that Bid has been shown to translocate to the mitochondria without cleavage in some model systems. The proteolytic cleavage of Bid usually occurs in the unstructured loop region between the alpha 2 and alpha 3 helices, which is between amino acids (aa) 41 and 79 of Bid. For example, the caspase-8/3 cleavage site is at 60 (human) and 59 (mouse). It should be noted that after cleavage, the smaller N-terminal portion of Bid is not necessarily separated from the larger C-terminal portion. The exact size of the C-terminal cleavage products depends on the Bid cleavage sites; generally 11-15 kD C-truncated cleavage forms are generated. Shorter C-terminal Bid cleavage products may result from protease cleavage that involves both caspases and other proteases. Full length Bid is quite stable, but cleaved/truncated forms may have a short half-life (less than 1.5 h) and degrade rapidly. Therefore, a time course may be useful when detecting Bid cleavage fragments.
Presentation
Antiserum, 0.05% sodium azide
Storage
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About BID
P55957 NM_001196 NP_001187.1

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Requested From: United States
Date Requested: 8/24/2019
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