Beta Amyloid 1-42

Mouse

Human, Mouse (tested or 100% immunogen sequence identity)

IgG2b Monoclonal

Ab5

Unconjugated

Protein G purified

Unmodified

Aggregated Aß1-42, fibrillar Aß.

• IHC - Paraffin (1 - 10 µg/ml)
• Immunofluorescence (1 - 10 µg/ml)
• Western blot (1 - 10 µg/ml)
• Immunoprecipitation (1 - 10 µg/ml)
• ELISA
• Sandwich ELISA

Immunoblotting, Immunohistochemistry: Immunofluorescence, Immunoprecipitation, Test at 1-10ug/ml in all applications. Sandwich ELISA as capture or HRPconjugated detection antibody. These are recommended concentrations; enduser should determine optimal concentrations for their applications. 96-well ELISA plates are coated with capture MAb at 2.5-5ug/well and incubated at 4oC overnight. The next day 300ul of blocking buffer is added, and plates are again incubated at 4 oC overnight. The next day plates are washed in PBS and serial dilution of Aß samples are added; plates are incubated overnight at 4oC. The next day plates are washed in PBS, and HRP-conjugated detection Aß MAb is added; plates are incubated for 4hrs at room temperature. Plates are washed in PBS-Tween and developed with TMB substrate for 5 mins. Antibody has only been tested in IHC and IF with formalin-fixed, paraffin-embedded samples.

PBS, pH 7.4

Store at -20°C for up to 1 year.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.