Human Beta Amyloid 1-42

Mouse

Human, Mouse (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

Ab42.2

Unconjugated

Protein G purified

Unmodified

Synthetic peptide corresponding to Aß35-42.

• IHC (1 - 10 µg/ml)
• Immunofluorescence (1 - 10 µg/ml)
• Western blot (1 - 10 µg/ml)
• Immunoprecipitation (1 - 10 µg/ml)
• ELISA
• Sandwich ELISA

Immunoblotting, Immunohistochemistry: Immunofluorescence, Immunoprecipitation Test at 1-10ug/ml. Sandwich ELISA (as capture antibody with #57004 as HRP-conjugated detection antibody). These are recommended concentrations; enduser should determine optimal concentrations for their applications. 96-well ELISA plates are coated with capture MAb at 2.5-5ug/well and incubated at 4oC overnight. The next day 300ul of blocking buffer is added, and plates are again incubated at 4 oC overnight. The next day plates are washed in PBS and serial dilution of Aß samples are added; plates are incubated overnight at 4oC. The next day plates are washed in PBS, and HRP-conjugated detection Aß MAb is added; plates are incubated for 4hrs at room temperature. Plates are washed in PBS-Tween and developed with TMB substrate for 5 mins.

PBS, pH 7.4

Store at -20°C for up to 1 year.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.