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Antibodies > BCL2L2 / Bcl-w > LS-C312586
 
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Catalog Number Size Price
LS-C312586-10 10 µg $318 
LS-C312586-100 100 µg (0.5 mg/ml) $470 
BCL2L2 / Bcl-w Antibody - IHC analysis of BCL2L2 using anti-BCL2L2 antibody. BCL2L2 was detected in paraffin-embedded section of human rectal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-BCL2L2 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
BCL2L2 / Bcl-w Antibody - Western blot analysis of Bcl2L2 using anti-Bcl2L2 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human U-937 whole cell lysates, Lane 4: human Hela whole cell lysates. , Lane 5: human A431 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Bcl2L2 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Bcl2L2 at approximately 21KD. The expected band size for Bcl2L2 is at 21KD.
BCL2L2 / Bcl-w Antibody - Flow Cytometry analysis of PC-3 cells using anti-BCL2L2 antibody. Overlay histogram showing PC-3 cells stained with anti-BCL2L2 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BCL2L2 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
BCL2L2 / Bcl-w Antibody - IHC analysis of BCL2L2 using anti-BCL2L2 antibody. BCL2L2 was detected in paraffin-embedded section of human rectal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-BCL2L2 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
BCL2L2 / Bcl-w Antibody - Western blot analysis of Bcl2L2 using anti-Bcl2L2 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human U-937 whole cell lysates, Lane 4: human Hela whole cell lysates. , Lane 5: human A431 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Bcl2L2 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Bcl2L2 at approximately 21KD. The expected band size for Bcl2L2 is at 21KD.
BCL2L2 / Bcl-w Antibody - Flow Cytometry analysis of PC-3 cells using anti-BCL2L2 antibody. Overlay histogram showing PC-3 cells stained with anti-BCL2L2 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BCL2L2 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Polyclonal Rabbit anti‑Human BCL2L2 / Bcl‑w Antibody (C‑Terminus, WB) LS‑C312586

Polyclonal Rabbit anti‑Human BCL2L2 / Bcl‑w Antibody (C‑Terminus, WB) LS‑C312586

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Antibody:
BCL2L2 / Bcl-w Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C312586-10
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
BCL2L2 / Bcl-w Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
Bcl-w antibody LS-C312586 is an unconjugated rabbit polyclonal antibody to Bcl-w (BCL2L2) (C-Terminus) from human. It is reactive with human, mouse and rat. Validated for WB.
Target
Human BCL2L2 / Bcl-w
Synonyms
BCL2L2 | BCLW | BCL2-L-2 | KIAA0271 | PPP1R51 | Apoptosis regulator Bcl-W | Bcl-2-like protein 2 | BCL-W | BCL2-like 2
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human BCL2L2(74-88 aa SAQQRFTQVSDELFQ), identical to the related rat and mouse sequences.
Epitope
C-Terminus
Specificity
Expressed (at protein level) in a wide range of tissues with highest levels in brain, spinal cord, testis, pancreas, heart, spleen and mammary glands. Moderate levels found in thymus, ovary and small intestine. Not detected in salivary gland, muscle or liver. Also expressed in cell lines of myeloid, fibroblast and epithelial origin. Not detected in most lymphoid cell lines. .
Applications
  • Western blot
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg Thimerosal, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About BCL2L2 / Bcl-w
Q92843 NM_004050 NP_004041.1

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PathPlus™ BCL2L2 / Bcl‑w Antibody (aa122‑171) LS‑B13998
Species: Mouse
Applications: IHC, IHC - Paraffin, Western blot
BCL2L2 / Bcl-w Antibody - Immunohistochemical analysis of BCLW staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
BCL2L2 / Bcl‑w Antibody (C‑Terminus) LS‑C353907
Species: Human, Mouse, Rat, Bovine, Dog
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
BCL2L2 / Bcl-w Antibody - Western blot testing of BCL2L2 antibody at 0.5ug/ml and HeLa lysate
BCL2L2 / Bcl‑w Antibody (aa74‑88) LS‑C388065
Species: Human, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Sheep
Applications: IHC, IHC - Paraffin, Western blot
BCL2L2 / Bcl-w Antibody - Western blot of Bcl-w antibody
BCL2L2 / Bcl‑w Antibody (100‑180 aa, C‑terminal) LS‑C382259
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UCHL1 / PGP9.5 Antibody - Anti-UCHL1 / PGP9.5 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
PathPlus™ UCHL1 / PGP9.5 Antibody (aa171‑220) LS‑B5981
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
See all BCL2L2 / Bcl-w Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 5/8/2024
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