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Antibodies > ATP6V1G3 > LS-C757453
 
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Catalog Number Size Price
LS-C757453-200 200 µl $393 
ATP6V1G3 Antibody - Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-2 OS (human osteosarcoma cell line) cells labeling ATP6V1G3 with ATP6V1G3 Antibody (N-Term) at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG (1583138) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm and weak nucleus staining on U-2 OS cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin (PD18466410) at 1/100 dilution (red).The nuclear counter stain is DAPI (blue).
ATP6V1G3 Antibody - All lanes: Anti-ATP6V1G3 Antibody (N-Term) at 1:2000 dilution Lane 1: Human kidney lysate Lane 2: Caki-1 whole cell lysate Lane 3: Renca whole cell lysate Lane 4: Mouse kidney lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 14 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
ATP6V1G3 Antibody - Overlay histogram showing U-2 OS cells stained with ATP6V1G3 Antibody (N-Term) (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (ATP6V1G3 Antibody (N-Term), 1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed (OE188374) at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG1 (1µg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
ATP6V1G3 Antibody - Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-2 OS (human osteosarcoma cell line) cells labeling ATP6V1G3 with ATP6V1G3 Antibody (N-Term) at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG (1583138) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm and weak nucleus staining on U-2 OS cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin (PD18466410) at 1/100 dilution (red).The nuclear counter stain is DAPI (blue).
ATP6V1G3 Antibody - All lanes: Anti-ATP6V1G3 Antibody (N-Term) at 1:2000 dilution Lane 1: Human kidney lysate Lane 2: Caki-1 whole cell lysate Lane 3: Renca whole cell lysate Lane 4: Mouse kidney lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 14 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
ATP6V1G3 Antibody - Overlay histogram showing U-2 OS cells stained with ATP6V1G3 Antibody (N-Term) (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (ATP6V1G3 Antibody (N-Term), 1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed (OE188374) at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG1 (1µg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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Polyclonal Rabbit anti‑Human ATP6V1G3 Antibody (N‑Terminus, IF, WB) LS‑C757453

Polyclonal Rabbit anti‑Human ATP6V1G3 Antibody (N‑Terminus, IF, WB) LS‑C757453

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Antibody:
ATP6V1G3 Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Application:
IF, WB, Flo, ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$393
LS-C757453-200
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
ATP6V1G3 Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Application:
IF, WB, Flo, ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
ATP6V1G3 antibody LS-C757453 is an unconjugated rabbit polyclonal antibody to ATP6V1G3 (N-Terminus) from human. It is reactive with human and mouse. Validated for ELISA, Flow, IF and WB.
Target
Human ATP6V1G3
Synonyms
ATP6V1G3 | ATP6G3 | V-ATPase G subunit 3 | Vma10 | V-ATPase 13 kDa subunit 3 | V-ATPase G3 subunit | V-ATPase subunit G 3
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Predicted
Xenopus (at least 90% immunogen sequence identity)
Clonality
Ig Polyclonal
Conjugations
Unconjugated
Purification
Protein A and Peptide Affinity Chromatography
Modifications
Unmodified
Immunogen
This ATP6V1G3 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 15-49 amino acids from the human region of human ATP6V1G3.
Epitope
N-Terminus
Applications
  • Immunofluorescence (1:25)
  • Western blot (1:2000)
  • Flow Cytometry (1:25)
  • ELISA
Usage
Western Blot Predicted Molecular Weight: 13.9 kDa
Presentation
PBS, 0.09% Sodium Azide
Storage
Short Term: Store at 2-8°C for up to 2 weeks. Long Term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ATP6V1G3
Q96LB4 NM_133262 NP_573569.1

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To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/28/2024
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