Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
ZAK is a MLK(MAP3K) type protein kinase that mediates the cell cycle signals leading to G2 arrest, possibly by regulating cyclin E expression. ZAK is activated in response to osmotic shock with hyperosmolar media through autophosphorylation. The expression of ZAK is increased by activation of the ERK/MAPK pathway and activates the ERK, JNK/SAPK, p38, and ERK5 pathways.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Western blot of anti-MLKLAK-C284 antibody in HepG2 cell line lysate (35 ug/lane). MLKLAK-C284(arrow) was detected using the purified antibody. This western blot detected isoform 2 of MLKLAK, the accession number for MLKLAK isoform 1 is Q9NYL2, NP_057737.