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Anti-Yeast Mer2 Antibody (phospho-Ser30) LS-C60090

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Wt. Vol. Conc. Price
100 µg - - $405
Inquire for larger quantities

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Related Products

Most Popular Yeast Mer2 Antibodies

Anti-Yeast Mer2 Antibody (phospho-Ser30) LS-C60090
Rabbit Polyclonal to Yeast Mer2
Yeast
Western blot, ELISA
Unconjugated
Western blot Image
Anti-Yeast Mer2 Antibody (aa26-35) LS-C60091
Rabbit Polyclonal to Yeast Mer2
Yeast
Western blot, ELISA
Unconjugated
Western blot Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Yeast Mer2
Yeast
Western blot, ELISA
Unconjugated

Details

Yeast Mer2
Rabbit
Yeast (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Protein A purified
Unmodified

Applications

  • Western blot (1:1000 - 1:10000)
  • ELISA (1:5000 - 1:25000)

Specificity and Use

Yeast Mer2 antibody was raised against synthetic peptide corresponding to amino acids 26-35 of Saccharomyces cerevisiae Mer2 protein.
pSer30
the phosphorylated form of Saccharomyces cerevisiae Mer2 protein at the pS30 residue
This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 50 kD in size corresponding to phosphorylated Mer2 protein by western blotting in the appropriate cell lysate or extract. Less than 2% reactivity is observed against the non-phosphorylated form of the immunizing peptide. This antibody is phospho specific for Mer2 phosphorylated at the pS30 residue. Preparation of extracts from cells 4hr after initiation of meiosis is suggested.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Long term: -20°C; Short term: -20°C
For research use only.

About Yeast Mer2


Western blot

Western blot
Anti-Mer2 pS30 Antibody - Western Blot. Western blot of affinity purified anti-S. cerevisiae Mer2 pS30 antibody shows detection of phos-phosphorylated Mer2 but not phosphatase treated or mutant cells. Lane 1 contains Mer2-myc protein detected in wild type cells after first immunoprecipitating the protein using anti-myc antibody. Cells were harvested 4 h after the initiation of meiosis and therefore contain mostly phosphorylated Mer2. Lane 2 contains the same preparation after treatment with phosphatase. Lane 3 contains Mer2-S30A protein as a phosphorylation control. This antibody appears to be specific for phosphorylated Mer2 at the S30 position with negligible cross reactivity against unphosphorylated protein. The primary antibody was used at a 1:5000 dilution. Personal Communication. Michael Lichten, NIH, CCR, Bethesda, MD.

Western blot

Western blot
Anti-Mer2 pS30 Antibody - Western Blot. Western blot of affinity purified anti-S. cerevisiae Mer2 pS30 antibody shows detection of phosphorylated Mer2 in whole cell extracts. Cells were either wild type (+) or contained mer2 deletions (D). Extracts were prepared from cells 4hr after initiation of meiosis. Proteins were obtained using TCA precipitation. The primary antibody was used at a 1:7500 dilution. Secondary antibody was used at 1:5000 dilution. Personal Communication. Michael Lichten, NIH, CCR, Bethesda, MD.

Requested From: United States
Date Requested: 12/3/2016

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