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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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XRCC1 Antibody (clone 2G8) LS‑C114944
XRCC1 antibody LS-C114944 is an unconjugated mouse monoclonal antibody to human XRCC1. Validated for Flow and WB.
Catalog
Size
Price
LS-C114944-100
100 µl (0.55 mg/ml)
Unavailable

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Product Description

XRCC1 antibody LS-C114944 is an unconjugated mouse monoclonal antibody to human XRCC1. Validated for Flow and WB.
About XRCC1
XRCC1 is involved in the efficient repair of DNA single-strand breaks formed by exposure to ionizing radiation and alkylating agents. This protein interacts with DNA ligase III, polymerase beta and poly (ADP-ribose) polymerase to participate in the base excision repair pathway. It may play a role in DNA processing during meiogenesis and recombination in germ cells. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. P18887 NM_006297 NP_006288.2

XRCC1 Antibody, RCC Antibody, DNA repair protein XRCC1 Antibody


Specifications

Target
Human XRCC1
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal [2G8]
Conjugations
Unconjugated
Purification
Protein A/G purified
Modifications
Unmodified
Applications
  • Western blot (1:2000)
  • Flow Cytometry (1:100)
Immunogen
XRCC1 antibody was raised against full length human recombinant protein of human XRCC1 (NP_006288) produced in HEK293T cell.
Specificity
Human XRCC1
Usage
Concentration: 0.5-1.0 mg/ml (Lot dependent)
Presentation
PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY XRCC1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-XRCC1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY XRCC1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-XRCC1.

Flow Cytometry

Flow cytometry of Jurkat cells, using anti-XRCC1 antibody, (Red) compared to a nonspecific negative control antibody (Blue).
Flow cytometry of Jurkat cells, using anti-XRCC1 antibody, (Red) compared to a nonspecific negative control antibody (Blue).

Flow Cytometry

Flow cytometry of HeLa cells, using anti-XRCC1 antibody, (Red) compared to a nonspecific negative control antibody (Blue).
Flow cytometry of HeLa cells, using anti-XRCC1 antibody, (Red) compared to a nonspecific negative control antibody (Blue).

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY XRCC1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-XRCC1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY XRCC1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-XRCC1.

Flow Cytometry

Flow cytometry of Jurkat cells, using anti-XRCC1 antibody, (Red) compared to a nonspecific negative control antibody (Blue).
Flow cytometry of Jurkat cells, using anti-XRCC1 antibody, (Red) compared to a nonspecific negative control antibody (Blue).

Flow Cytometry

Flow cytometry of HeLa cells, using anti-XRCC1 antibody, (Red) compared to a nonspecific negative control antibody (Blue).
Flow cytometry of HeLa cells, using anti-XRCC1 antibody, (Red) compared to a nonspecific negative control antibody (Blue).

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY XRCC1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-XRCC1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY XRCC1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-XRCC1.

Flow Cytometry

Flow cytometry of Jurkat cells, using anti-XRCC1 antibody, (Red) compared to a nonspecific negative control antibody (Blue).
Flow cytometry of Jurkat cells, using anti-XRCC1 antibody, (Red) compared to a nonspecific negative control antibody (Blue).

Flow Cytometry

Flow cytometry of HeLa cells, using anti-XRCC1 antibody, (Red) compared to a nonspecific negative control antibody (Blue).
Flow cytometry of HeLa cells, using anti-XRCC1 antibody, (Red) compared to a nonspecific negative control antibody (Blue).

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY XRCC1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-XRCC1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY XRCC1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-XRCC1.

Flow Cytometry

Flow cytometry of Jurkat cells, using anti-XRCC1 antibody, (Red) compared to a nonspecific negative control antibody (Blue).
Flow cytometry of Jurkat cells, using anti-XRCC1 antibody, (Red) compared to a nonspecific negative control antibody (Blue).

Flow Cytometry

Flow cytometry of HeLa cells, using anti-XRCC1 antibody, (Red) compared to a nonspecific negative control antibody (Blue).
Flow cytometry of HeLa cells, using anti-XRCC1 antibody, (Red) compared to a nonspecific negative control antibody (Blue).


Requested From: 
Date Requested: 6/19/2018

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