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Anti-WRNIP1 / WHIP Antibody (Internal) IHC-plus™ LS-B436

Note: This antibody replaces LS-C19088

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular WRNIP1 / WHIP Antibodies

Anti-WRNIP1 / WHIP Antibody (Internal) IHC-plus™ LS-B436
Rabbit Polyclonal to Human WRNIP1 / WHIP
Human, Monkey, Mouse, Rat
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-WRNIP1 / WHIP Antibody (aa67-116) IHC-plus™ LS-B683
Rabbit Polyclonal to Human WRNIP1 / WHIP
Human, Monkey, Mouse, Rat, Bat, Bovine, Hamster
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-WRNIP1 / WHIP Antibody (aa1-30, Azide-free, HRP) LS-C247259
Rabbit Polyclonal (IgG) to Human WRNIP1 / WHIP
Human
Western blot, ELISA
HRP Conjugated, Azide-free

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human WRNIP1 / WHIP
Human, Monkey, Mouse, Rat
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human WRNIP1 / WHIP
Rabbit
Human, Monkey, Mouse, Rat (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (2.5 µg/ml)
  • Western blot (1:500 - 1:2000)
  • ELISA (1:10000 - 1:40000)

Specificity and Use

WRNIP1 / WHIP antibody was raised against synthetic peptide from human WRNIP1 / WHIP.
Internal
An internal region of the WHIP1 protein. The immunogen sequence shows 100% homology to human WHIP1 (isoform 1) and WHIP2 (isoform 2) with predicted molecular weights of 72.2 kD and 69.5 kD, respectively. The immunogen sequence also shows 100% homology to WHIP1 from mouse, rat and monkey sequences. Reactivity with WHIP proteins from other sources is not known, but is likely due to reported homologies.
Immunohistochemistry: LS-B436 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B436 was determined to be 2.5 ug/ml.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

About WRNIP1 / WHIP

Q96S55 NM_020135 NP_064520.2

WRNIP1 Antibody, Putative helicase RUVBL Antibody, WHIP Antibody, RP11-420G6.2 Antibody, ATPase WRNIP1 Antibody, BA420G6.2 Antibody

Werner's syndrome is a rare autosomal recessive disorder characterized by accelerated aging that is caused by defects in the Werner syndrome ATP-dependent helicase gene (WRN). The protein encoded by this gene interacts with the exonuclease-containing N-terminal portion of the Werner protein. This protein has a ubiquitin-binding zinc-finger domain in the N-terminus, an ATPase domain, and two leucine zipper motifs in the C-terminus.

Immunohistochemistry

Immunohistochemistry
Anti-WRNIP1 / WHIP antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B436 concentration 5 ug/ml.

Western blot

Western blot
Anti-Human WHIP Antibody - Western Blot. Western blot analysis is shown using anti-Human WHIP antibody with and without pre-incubation with blocking peptide. Testing was performed on antiserum prior to affinity purification. Peptide competition (left) blocks the specific staining, whereas the control (right) shows staining of a strong dominant band corresponding to human WHIP1. ~30 ug of HEK293 lysate was loaded per lane for 4-20% gradient SDS-PAGE. Comparison to a molecular weight marker (not shown) indicates a band of ~96.0 kD is detected. The blot was incubated with a 1:1000 dilution of the antibody at room temperature for 2 h followed by detection using IRDye 800 labeled Goat-a-Rabbit IgG [H&L] MX10 ( diluted 1:5000 for 45 min. IRDye 800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other systems will yield similar results.

Western blot

Western blot
Anti-Human WHIP Antibody - Western Blot. Western blot analysis is shown using Affinity Purified anti-Human WHIP antibody to detect Human WHIP present in a HEK293 whole cell lysate. ~30 ug of lysate was loaded per lane for 4-20% gradient SDS-PAGE. Comparison to a molecular weight marker (not shown) indicates a primary band of ~96.0 kD is detected. The identity of the minor band migrating at a slightly higher molecular weight is unknown, but may represent an alternate isoform of WHIP or post translational modification of the WHIP protein. See Figure 2 for the results of peptide competition experiments. The blot was incubated with a 1:200 dilution of the antibody at room temperature for 2 h followed by detection using IRDye 800 labeled Goat-a-Rabbit IgG [H&L] MX10 ( diluted 1:5000 for 45 min. IRDye 800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
 

Requested From: United States
Date Requested: 12/6/2016

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