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Catalog Number Size Price
LS-C60118-100 100 µg (1 mg/ml) $567 
WNT1 Antibody - Anti-Wnt1 Antibody - Western Blot. Western blot of affinity purified anti-Wnt1 antibody shows detection of endogenous Wnt1 in human-derived MCF7 cell lysate (lane 1) and mouse-derived 3T3 cell lysate (lane 2). The band at ~41 kD, indicated by the arrowhead, corresponds to Wnt1. After transfer, the membrane was blocked with 5% BLOTTO. Primary antibody was used at a 1:1400 dilution in PBS containing 1% BLOTTO. The specificity of the antibody was confirmed by peptide competition which completely blocked reaction of the antibody with Wnt1 (data not shown).

Polyclonal Rabbit anti‑Human WNT1 Antibody (WB) LS‑C60118

Polyclonal Rabbit anti‑Human WNT1 Antibody (WB) LS‑C60118

Antibody:
WNT1 Rabbit anti-Human Polyclonal Antibody
Application:
WB, ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$567
LS-C60118-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
WNT1 Rabbit anti-Human Polyclonal Antibody
Application:
WB, ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
WNT1 antibody LS-C60118 is an unconjugated rabbit polyclonal antibody to WNT1 from human. It is reactive with human and mouse. Validated for ELISA and WB.
Target
Human WNT1
Synonyms
WNT1 | INT1 | Proto-oncogene Int-1 homolog | Proto-oncogene Wnt-1
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human Wnt1 protein.
Specificity
This antibody reacts with human and mouse Wnt1 protein. A BLAST analysis was used to suggest cross-reactivity with Wnt1 from mouse, human, rat, bovine, dog, macaque, and opossum based on a 100% homology with the immunizing sequence. Partial cross-reactivity is expected against chicken Wnt1 based on a 91% sequence homology. Cross-reactivity with Wnt1 from other sources has not been determined.
Applications
  • Western blot (1:1500 - 1:6000)
  • ELISA (1:25000)
Usage
This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~41 kD in size corresponding to Wnt1 by western blotting in the appropriate cell lysate or extract. This antibody is capable of detecting endogenous Wnt1.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: -20°C; Long term: -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About WNT1
P04628 NM_005430 NP_005421.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/26/2024