Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human, Monkey, Mouse, Rat, Dog, Goat, Pig, Chicken, Zebrafish
IHC, IHC - Frozen, ICC, Flow Cytometry
Mouse Monoclonal [clone VI-10] (IgM) to Human Vimentin
Human, Mouse, Rat, Pig, Chicken
IHC, IHC - Paraffin, ICC, Western blot, Immunoprecipitation
Human, Mouse, Rat, Pig, Chicken (tested or 100% immunogen sequence identity)
IgM Monoclonal [VI-10]
Caprylic acid and ammonium sulfate precipitation
IHC - Paraffin (5 µg/ml)
Specificity and Use
Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody,
PBS, 15 mM sodium azide, approx., pH 7.4. Sourced in Ascites.
Vimentin is a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification.