Human Vimentin

Mouse

Human, Bovine, Dog, Goat, Pig, Chicken (tested or 100% immunogen sequence identity)

Mouse, Rat

IgG1 Monoclonal

SPM576

Unconjugated

Protein G purified

Unmodified. Also available Azide-free, BSA-free.

Human recombinant protein.

Human Vimentin

• IHC
• IHC - Paraffin (0.25 - 0.5 µg/ml)
• Immunofluorescence (1 - 2 µg/ml)
• Western blot (0.25 - 0.5 µg/ml)
• Flow Cytometry (0.5 - 1 µg/10E6 cells)

This mAb reacts with a 58kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFPs) such as desmin, keratin, neurofilament, and glial fibrillary acid protein. Anti-vimentin alone is of limited value as a diagnostic tool. however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent. The optimal dilution of the VIM antibody for each application should be determined by the researcher. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

1X PBS, 0.1 mg/ml BSA, 0.05% sodium azide

Store at 2°C to 8°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P08670 NCBI: NM_003380 NP_003371.2