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Catalog Number Size Price
LS-C204599-100 100 µl (1 mg/ml) $376 
Vimentin Antibody - View of mixed neuron/glial cultures stained with Vimentin antibody (green) our our rabbit antibody to GFAP antibody (RPCA-GFAP, red). Vimentin is expressed alone in fibroblastic and endothelial cells, which are the flattened cells in the middle of the image which appear green. Astrocytes may express primarily GFAP, or GFAP and vimentin, and so appear red (GFAP only) or golden yellow (GFAP and Vimentin). In cells which express both GFAP and vimentin, the two protein assemble to produce heteropolymer filaments.
Vimentin Antibody - Western blot of crude extract of the human carcinoma MCA-2A52 clone. Lane 15 was probed with our antibody to actin, giving an SDS-PAGE molecular weight of 42kDa, and lane 16 with MCA-3G12, our antibody to 14-3-3 eta, which has an SDS-PAGE molecular weight of 28kDa.
Vimentin Antibody - View of mixed neuron/glial cultures stained with Vimentin antibody (green) our our rabbit antibody to GFAP antibody (RPCA-GFAP, red). Vimentin is expressed alone in fibroblastic and endothelial cells, which are the flattened cells in the middle of the image which appear green. Astrocytes may express primarily GFAP, or GFAP and vimentin, and so appear red (GFAP only) or golden yellow (GFAP and Vimentin). In cells which express both GFAP and vimentin, the two protein assemble to produce heteropolymer filaments.
Vimentin Antibody - Western blot of crude extract of the human carcinoma MCA-2A52 clone. Lane 15 was probed with our antibody to actin, giving an SDS-PAGE molecular weight of 42kDa, and lane 16 with MCA-3G12, our antibody to 14-3-3 eta, which has an SDS-PAGE molecular weight of 28kDa.
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Monoclonal Mouse anti‑Human Vimentin Antibody (clone 2D1, IF, WB) LS‑C204599

Monoclonal Mouse anti‑Human Vimentin Antibody (clone 2D1, IF, WB) LS‑C204599

Antibody:
Vimentin Mouse anti-Human Monoclonal (2D1) Antibody
Application:
ICC, IF, WB
Reactivity:
Human, Mouse, Rat, Bovine, Pig
Format:
Unconjugated, Unmodified
Price
Catalog Number
$376
LS-C204599-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
Vimentin Mouse anti-Human Monoclonal (2D1) Antibody
Application:
ICC, IF, WB
Reactivity:
Human, Mouse, Rat, Bovine, Pig
Format:
Unconjugated, Unmodified

Specifications

Description
Vimentin antibody LS-C204599 is an unconjugated mouse monoclonal antibody to Vimentin from human. It is reactive with human, mouse, rat and other species. Validated for ICC, IF and WB.
Target
Human Vimentin
Synonyms
VIM | Vimentin | vimenin
Host
Mouse
Reactivity
Human, Mouse, Rat, Bovine, Pig (tested or 100% immunogen sequence identity)
Clonality
IgG2a,k Monoclonal
Clone
2D1
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
The immunogen used to generate our antibody was recombinant human vimentin expressed in and purified from E. coli.
Specificity
Clone 12D1 is known to react with vimentin from human, cow, pig, mouse, rat and other mammals. Since vimentin is highly conserved, it is likely that the antibody is effective on other species also.
Applications
  • ICC (1:1000)
  • Immunofluorescence (1:1000)
  • Western blot (1:10000)
Usage
The antibody solution can be used at dilutions of at least 1:1000 in immunofluorescence experiments. In western blotting using chemiluminescence it can be used at dilutions of 1:10000 or lower.
Presentation
PBS, 10 mM Sodium Azide
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About Vimentin
P08670 NM_003380 NP_003371.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 9/18/2024