Target
Human UMOD / Uromodulin
Synonyms
UMOD | ADMCKD2 | FJHN | HNFJ | MCKD2 | THGP | THP | Uromucoid | Tamm-Horsfall glycoprotein | HNFJ1 | Uromodulin
Reactivity
Human, Monkey
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence C-PITRKGVQATVS, from the C-Terminus of protein sequence according to NP_003352.2.
Specificity
Human UMOD / Uromodulin. Reported variants represent identical protein: NP_001008390.1, NP_003352.2; The immunizing peptide represents the C terminus of the mature protein.
Applications
- Western blot (0.03 - 0.1 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:128000)
Usage
Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 65kD band observed in lysates of cell line HeLa (calculated MW of 69.8kD according to NP_003352.2). Recommended concentration: 0.03-0.1 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee