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Anti-UGT8 Antibody (aa525-535) LS-C154805

Ordering

Wt. Vol. Conc. Price
100 µg - 0.5 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular UGT8 Antibodies

Anti-UGT8 Antibody (aa373-401, AP) LS-C273022
Rabbit Polyclonal (IgG) to Mouse UGT8
Mouse
IHC, Western blot, ELISA
AP Conjugated
Anti-UGT8 Antibody (aa373-401, FITC) LS-C273025
Rabbit Polyclonal (IgG) to Mouse UGT8
Mouse
IHC, Western blot, ELISA
FITC Conjugated
Anti-UGT8 Antibody (aa198-541, FITC) LS-C301887
Rabbit Polyclonal (IgG) to Human UGT8
Human
Western blot, ELISA
FITC Conjugated
Western blot Image

100% Guaranteed 100% Guaranteed
Goat Polyclonal to Human UGT8
Human, Monkey, Bovine, Dog, Guinea pig, Horse, Rabbit
Western blot, ELISA
Unconjugated

Details

Human UGT8
Goat
Human, Monkey, Bovine, Dog, Guinea pig, Horse, Rabbit (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • Western blot (1 - 3 µg/ml)
  • ELISA (1:64000)

Specificity and Use

UGT8 antibody was raised against synthetic peptide C-NGKYKRNGHIK from an internal region of human UGT8 (NP_003351.2). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Dog, Bovine, Elephant, Panda, Horse, Rabbit, Porcine, Opossum, Guinea pig (100%); Zebra finch, Platypus, Lizard (91%); Rat, Turkey, Chicken, Xenopus, Zebrafish (82%).
LS-E27546 - Lyophilized - 100 µg - $145.00
aa525-535
Human UGT8. Reported variants represent identical protein: NP_001121646.1, NP_003351.2.
Peptide ELISA: antibody detection limit dilution 1:64000. Western blot: In transfected MCF7 transiently expressing Human UGT8 a band of approx. 65kD is observed. This band is not observed in the non-transfected MCF7. The calculated molecular size is 61.5kD according to NP_003351.2. Recommended concentration, 1-3 ug/ml. Preliminary experiments gave an approx 50kD band in Human -Brain lysates and 48KDa in Rat Brain lysates after 1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 61.5kD according to NP_003351.2. The observed band was successfully blocked by incubation with the immunizing peptide.

Packaging

Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Store at -20°C. Minimize freezing and thawing.
For research use only.

About UGT8

Q16880 NM_003360 NP_003351.2

UGT8 Antibody, Cerebroside synthase Antibody, CGT Antibody, UGT4 Antibody, UDP glycosyltransferase 8 Antibody

Catalyzes the transfer of galactose to ceramide, a key enzymatic step in the biosynthesis of galactocerebrosides, which are abundant sphingolipids of the myelin membrane of the central nervous system and peripheral nervous system.

Western blot

Western blot
UGT8 antibody MCF7 overexpressing Human UGT8 and probed with (mock transfection in first lane). Primary incubation (1 ug/ml) was overnight at 4C. Data provided by Tomasz Owczarek, Wroclaw University, Poland. Detected by chemiluminescence.
 

Requested From: United States
Date Requested: 12/7/2016

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