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Catalog Number Size Price
LS-C154805-100 100 µg (0.5 mg/ml) $503 
UGT8 Antibody - UGT8 antibody MCF7 overexpressing Human UGT8 and probed with (mock transfection in first lane). Primary incubation (1 ug/ml) was overnight at 4C. Data provided by Tomasz Owczarek, Wroclaw University, Poland. Detected by chemiluminescence.

Polyclonal Goat anti‑Human UGT8 Antibody (aa525‑535, WB) LS‑C154805

Polyclonal Goat anti‑Human UGT8 Antibody (aa525‑535, WB) LS‑C154805

Antibody:
UGT8 Goat anti-Human Polyclonal (aa525-535) Antibody
Application:
WB, Peptide-ELISA
Reactivity:
Human, Monkey, Bovine, Dog, Guinea pig, Horse, Rabbit
Format:
Unconjugated, Unmodified
Price
Catalog Number
$503
LS-C154805-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
UGT8 Goat anti-Human Polyclonal (aa525-535) Antibody
Application:
WB, Peptide-ELISA
Reactivity:
Human, Monkey, Bovine, Dog, Guinea pig, Horse, Rabbit
Format:
Unconjugated, Unmodified

Specifications

Description
UGT8 antibody LS-C154805 is an unconjugated goat polyclonal antibody to UGT8 (aa525-535) from human. It is reactive with human, bovine, dog and other species. Validated for Peptide-ELISA and WB.
Target
Human UGT8
Synonyms
UGT8 | Cerebroside synthase | CGT | UGT4 | UDP glycosyltransferase 8
Host
Goat
Reactivity
Human, Monkey, Bovine, Dog, Guinea pig, Horse, Rabbit (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Immunogen
Peptide with sequence C-NGKYKRNGHIK, from the internal region of the protein sequence according to NP_003351.2.
Epitope
aa525-535
Specificity
Human UGT8. Reported variants represent identical protein: NP_001121646.1, NP_003351.2.
Applications
  • Western blot (1 - 3 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:64000)
Usage
Peptide ELISA: antibody detection limit dilution 1:64000. Western blot: In transfected MCF7 transiently expressing Human UGT8 a band of approx. 65kD is observed. This band is not observed in the non-transfected MCF7. The calculated molecular size is 61.5kD according to NP_003351.2. Recommended concentration, 1-3 ug/ml. Preliminary experiments gave an approx 50kD band in Human -Brain lysates and 48KDa in Rat Brain lysates after 1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 61.5kD according to NP_003351.2. The observed band was successfully blocked by incubation with the immunizing peptide.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About UGT8
Q16880 NM_003360 NP_003351.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

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Requested From: United States
Date Requested: 4/26/2024