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Anti-UCHL1 / PGP9.5 Antibody LS-C204591

Ordering

Wt. Vol. Conc. Price
- 100 µl - $285
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular UCHL1 / PGP9.5 Antibodies

Anti-UCHL1 / PGP9.5 Antibody LS-C122356
Rabbit Polyclonal (IgG) to Human UCHL1 / PGP9.5
Human, Mouse, Rat, Bovine, Cat, Dog, Guinea pig, Hamster, Pig, Rabbit, Sheep
IHC - Paraffin, Western blot
Unconjugated
Anti-UCHL1 / PGP9.5 Antibody (aa171-220) IHC-plus™ LS-B5981
Rabbit Polyclonal (IgG) to Human UCHL1 / PGP9.5
Human, Mouse, Rat
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Chicken Polyclonal (IgY) to Human UCHL1 / PGP9.5
Human
ICC, Immunofluorescence, Western blot
Unconjugated

Details

Human UCHL1 / PGP9.5
Chicken
Human (tested or 100% immunogen sequence identity)
IgY Polyclonal
Unconjugated
Purified
Unmodified

Applications

  • ICC (1:1000)
  • Immunofluorescence (1:1000)
  • Western blot (1:10000)

Specificity and Use

UCHL1 / PGP9.5 antibody was raised against recombinant full length human UCHL1 purified from E. coli.
Human UCHL1 / PGP9.5
Try at dilutions of 1:1000 and higher for immunofluorescence. For western blots try at 1:10000. A suitable control tissue is rat spinal cord or peripheral nerve homogenate. The UCHL1 protein runs at about 27kDa on SDS-PAGE gels, and is a prominent component of brain, spinal cord and especially cortical extracts.

Packaging

Liquid
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

About UCHL1 / PGP9.5

P09936 NM_004181 NP_004172.2

UCHL1 Antibody, Ubiquitin C-terminal hydrolase Antibody, PARK5 Antibody, PGP9.5 Antibody, PGP95 Antibody, Ubiquitin thioesterase L1 Antibody, Neuron cytoplasmic protein 9.5 Antibody, PGP 9.5 Antibody, Uch-L1 Antibody

Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.

Immunocytochemistry

Immunocytochemistry
Rat mixed neuron/glial cultures stained with chicken UCHL1 (green) and rabbit antibody to glial fibrillary acidic protein (GFAP-red). Blue is a DNA stain. Note that the UCHL1 stains neurons strongly and specifically, and that the staining is concentrated in the cell bodies, though some does extend into the dendrites also.

Western blot

Western blot
Blots of whole cell homogenate of the human SH-SY5Y neuroblastoma cell line stained with chicken antibody to glyceraldehyde 3 phosphate dehydrogenase (GAPDH, left lane, blot made with 1D4 monoclonal antibody to GAPDH) and UCHL1 / PGP9.5 antibody (right lane). The GAPDH antibody stains a ~37kDa band while the UCHL1 antibody stains a band at about 24kDa.

Requested From: United States
Date Requested: 12/4/2016

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