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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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UBE2I / UBC9 Antibody (aa2‑14) IHC‑plus™ LS‑B6718
Note: This antibody replaces LS-C20222
UBC9 antibody LS-B6718 is an unconjugated goat polyclonal antibody to UBC9 (UBE2I) from human, mouse, rat and other species. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B6718-50
50 µg (0.5 mg/ml)
Unavailable

Popular UBE2I / UBC9 Products

Western blot of anti-UBC9 Antibody in mouse spleen tissue lysates (35 ug/lane). UBC9(arrow) was detected using the purified antibody.
Species: Human, Mouse
Applications: Western blot
Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE)
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry, ELISA
Immunohistochemistry of paraffin-embedded human esophageal cancer using UBE2I antibody at dilution of 1:200 (400x lens).
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot
Antibody
Species: Human
Applications: IHC, Immunofluorescence, Western blot, Flow Cytometry, ELISA

Product Description

UBC9 antibody LS-B6718 is an unconjugated goat polyclonal antibody to UBC9 (UBE2I) from human, mouse, rat and other species. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
About UBE2I / UBC9
Accepts the ubiquitin-like proteins SUMO1, SUMO2, SUMO3 and SUMO4 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2 or CBX4. Can catalyze the formation of poly-SUMO chains. Necessary for sumoylation of FOXL2 and KAT5. Essential for nuclear architecture and chromosome segregation. Sumoylates p53/TP53 at 'Lys-386'. P63279 NM_003345 NP_003336.1

UBE2I Antibody, C358B7.1 Antibody, SUMO-conjugating enzyme UBC9 Antibody, UBC9 Antibody, SUMO-protein ligase Antibody, UBCE9 Antibody, Ubiquitin carrier protein 9 Antibody, Ubiquitin carrier protein I Antibody, Ubiquitin-protein ligase I Antibody, p18 Antibody, SUMO-1-protein ligase Antibody, Ubiquitin conjugating enzyme 9 Antibody, Ubiquitin-protein ligase E2I Antibody


Specifications

Target
Human UBE2I / UBC9
Host
Goat
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Hamster, Horse, Rabbit, Chicken, Xenopus, Zebrafish (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.1 - 0.3 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:64000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
UBE2I / UBC9 antibody was raised against synthetic peptide SGIALSRLAQERK-C from the N-terminus of human UBE2I / UBC9 (NP_003336.1; NP_919235.1; NP_919236.1; NP_919237.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Bat, Horse, Rabbit, Opossum, Turkey, Chicken, Xenopus, Trout, Seabass, Catfish, Salmon, Smelt, Sablefish, Stickleback, Zebrafish (100%); Frog, Pufferfish (92%); Sea anemone, Slime mold (85%).
Blocking Peptide
LS-E25472 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B6718. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa2-14
Specificity
Human UBE2I / UBC9. This antibody is expected to recognize all reported isoforms (NP_003336.1, NP_919235.1, NP_919236.1 and NP_919237.1).
Presentation
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Storage
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Anti-UBE2I antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6718 concentration 5 ug/ml.
Anti-UBE2I antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6718 concentration 5 ug/ml.

Western blot

(0.1 ug/ml) Staining of Human Kidney lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
(0.1 ug/ml) Staining of Human Kidney lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry

Anti-UBE2I antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6718 concentration 5 ug/ml.
Anti-UBE2I antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6718 concentration 5 ug/ml.

Western blot

(0.1 ug/ml) Staining of Human Kidney lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
(0.1 ug/ml) Staining of Human Kidney lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry

Anti-UBE2I antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6718 concentration 5 ug/ml.
Anti-UBE2I antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6718 concentration 5 ug/ml.

Western blot

(0.1 ug/ml) Staining of Human Kidney lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
(0.1 ug/ml) Staining of Human Kidney lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry

Anti-UBE2I antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6718 concentration 5 ug/ml.
Anti-UBE2I antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6718 concentration 5 ug/ml.

Western blot

(0.1 ug/ml) Staining of Human Kidney lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
(0.1 ug/ml) Staining of Human Kidney lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.


Requested From: 
Date Requested: 6/24/2018

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