Target
Lima Bean Trypsin Inhibitor
Reactivity
Lima Bean
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
Modifications
Lyophilized
Immunogen
Trypsin Inhibitor [Lima Bean].
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Trypsin Inhibitor [Lima Bean]. Cross reactivity against Trypsin Inhibitor from other tissues and species may occur but have not been specifically determined.
Applications
- Western blot (1:500 - 1:3000)
- ELISA (1:5000 - 1:25000)
Usage
TRYPSIN INHIBITOR antibody has been assayed against 1.0 ug of Trypsin Inhibitor [Lima Bean] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1:4000 to 1:15000 of the reconstitution concentration is suggested for this product.
Presentation
Lyophilized from 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitution
Reconstitute in 100 µL of deionized water.
Storage
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee