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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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Catalog Number Size Price
LS-C153797-100 100 µg (1 mg/ml) $425 
Trypsin Inhibitor Antibody - Rabbit anti Soybean Trypsin Inhibitor antibody was used to detect purified Soybean Trypsin Inhibitor. Samples of ~1 and 0.25 ug of purified Soybean Trypsin Inhibitor per lane were run by SDS-PAGE under reducing and non-reducing conditions and reduced samples of protein contained 4% BME and were boiled for 5 minutes. Protein was transferred to nitrocellulose and probed with Rabbit anti Soybean Trypsin Inhibitor antibody (200-4679 lot 6594 1:5K in MB-0070, ON 4 C). Primary antibody was detected with Dylight 488 conjugated Streptavidin (S000-41 Lot 21091, 1:5K) and Atto 425 conjugated goat anti rabbit (LS-C153934 lot 26423C 1:10K 1.5 hr RT in MB-070) and imaged on the BioRad VersaDoc imaging system.

Polyclonal Rabbit anti‑Soy Bean Trypsin Inhibitor Antibody (WB) LS‑C153797

Polyclonal Rabbit anti‑Soy Bean Trypsin Inhibitor Antibody (WB) LS‑C153797

Antibody:
Rabbit Polyclonal to Soy Bean Trypsin Inhibitor
Application:
WB, IP, ELISA
Reactivity:
Soy Bean
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-C153797-100
100 µg
1 mg/ml
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Rabbit Polyclonal to Soy Bean Trypsin Inhibitor
Application:
WB, IP, ELISA
Reactivity:
Soy Bean
Format:
Unconjugated, Unmodified

Specifications

Description
Trypsin Inhibitor antibody LS-C153797 is an unconjugated rabbit polyclonal antibody to soy bean Trypsin Inhibitor. Validated for ELISA, IP and WB.
Target
Soy Bean Trypsin Inhibitor
Host
Rabbit
Reactivity
Soy Bean (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
Modifications
Unmodified
Applications
  • Western blot (1:500 - 1:5000)
  • Immunoprecipitation (1:100)
  • ELISA (1:5000 - 1:20000)
Immunogen
Trypsin Inhibitor [Soybean].
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Trypsin Inhibitor [Soybean]. Cross reactivity against Trypsin Inhibitor from other sources may occur but have not been specifically determined.
Usage
This product has been assayed against 1.0 ug of Trypsin Inhibitor [Soy Bean] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:3000 to 1:12000 of the reconstitution concentration is suggested for this product. This product has been assayed against 1.0 ug of Trypsin Inhibitor [Soy Bean] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:3000 to 1:12000 of the reconstitution concentration is suggested for this product. This product has been assayed against 1.0 ug of Trypsin Inhibitor [Soy Bean] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:3000 to 1:12000 of the reconstitution concentration is suggested for this product. This product has been assayed against 1.0 ug of Trypsin Inhibitor [Soy Bean] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:3000 to 1:12000 of the reconstitution concentration is suggested for this product.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Requested From: United States
Date Requested: 6/4/2020