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Trypsin Inhibitor Antibody LS‑C153797

Catalog Number Size Price
LS-C153797-100 100 µg (1 mg/ml) $425 
Rabbit anti Soybean Trypsin Inhibitor antibody was used to detect purified Soybean Trypsin Inhibitor. Samples of ~1 and 0.25 ug of purified Soybean Trypsin Inhibitor per lane were run by SDS-PAGE under reducing and non-reducing conditions and reduced samples of protein contained 4% BME and were boiled for 5 minutes. Protein was transferred to nitrocellulose and probed with Rabbit anti Soybean Trypsin Inhibitor antibody (200-4679 lot 6594 1:5K in MB-0070, ON 4 C). Primary antibody was detected with Dylight 488 conjugated Streptavidin (S000-41 Lot 21091, 1:5K) and Atto 425 conjugated goat anti rabbit (LS-C153934 lot 26423C 1:10K 1.5 hr RT in MB-070) and imaged on the BioRad VersaDoc imaging system.

Trypsin Inhibitor Antibody LS‑C153797

Antibody:
Rabbit Polyclonal to Soybean Trypsin Inhibitor
Application:
WB, IP, ELISA
Reactivity:
Soybean
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-C153797-100
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Soybean Trypsin Inhibitor
Application:
WB, IP, ELISA
Reactivity:
Soybean
Format:
Unconjugated, Unmodified

Specifications

Description
Trypsin Inhibitor antibody LS-C153797 is an unconjugated rabbit polyclonal antibody to soybean Trypsin Inhibitor. Validated for ELISA, IP and WB.
Target
Soybean Trypsin Inhibitor
Host
Rabbit
Reactivity
Soybean (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Ion exchange chromatography
Modifications
Unmodified
Applications
  • Western blot (1:500 - 1:5000)
  • Immunoprecipitation (1:100)
  • ELISA
Immunogen
Trypsin Inhibitor [Soy Bean].
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Trypsin Inhibitor [Soy Bean]. Cross reactivity against Trypsin Inhibitor from other sources may occur but have not been specifically determined.
Usage
This product has been assayed against 1.0 ug of Trypsin Inhibitor [Soy Bean] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:3000 to 1:12000 of the reconstitution concentration is suggested for this product. This product has been assayed against 1.0 ug of Trypsin Inhibitor [Soy Bean] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:3000 to 1:12000 of the reconstitution concentration is suggested for this product. This product has been assayed against 1.0 ug of Trypsin Inhibitor [Soy Bean] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:3000 to 1:12000 of the reconstitution concentration is suggested for this product. This product has been assayed against 1.0 ug of Trypsin Inhibitor [Soy Bean] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:3000 to 1:12000 of the reconstitution concentration is suggested for this product.
Presentation
Sterile Solution 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Featured Products

Western Blot of Rabbit Anti Trypsin Inhibitor antibody. Lane 1: purified Soybean Trypsin Inhibitor Reduced. Lane 2: purified Soybean Trypsin Inhibitor Non-Reduced. Lane 3: purified Streptavidin Reduced. Lane 4: purified Streptavidin Non-Reduced. Load: 1.0 ug per lane. Primary antibody: Biotin conjugated Rabbit anti-trypsin inhibitor antibody and Rabbit anti streptavidin 1:1000 for overnight at 4 degrees C. Secondary antibody: Dylight 649 conjugated Donkey anti rabbit at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4 degrees C. Predicted/Observed size: 25 kDa for Trypsin Inhibitor. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Soy Bean
Applications: IHC, Western blot, ELISA
Reactivity: Human
Range: 0.156-10 ng/ml
Anti-AGTR1 antibody IHC of human kidney, vessel. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Product
Species: Human, Mouse, Rat, Bovine
Applications: Immunofluorescence
Reactivity: Human
Range: 7.8-500 pmol/ml

Requested From: United States
Date Requested: 8/25/2019
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