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Anti-TRIM1 / MID2 Antibody (aa723-735) IHC-plus™ LS-B2277

Note: This antibody replaces LS-C54779, LS-C66390
Catalog Size Price
LS-B2277-50 50 µg (0.5 mg/ml) Unavailable

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Anti-TRIM1 / MID2 Antibody (aa723-735) IHC-plus™ LS-B2277
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Human, Monkey, Mouse
IHC - Paraffin, Western blot, ELISA
Immunohistochemistry Image
Anti-TRIM1 / MID2 Antibody (aa690-717, AP) LS-C237638
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Anti-TRIM1 / MID2 Antibody (aa669-698) LS-C323004
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100% Guaranteed
Goat Polyclonal to Human TRIM1 / MID2
Human, Monkey, Mouse
IHC - Paraffin, Western blot, ELISA


Human TRIM1 / MID2
Human, Monkey, Mouse (tested or 100% immunogen sequence identity)
Rat, Bat, Dog, Horse, Pig, Chicken (at least 90% immunogen sequence identity)
Immunoaffinity purified


  • IHC - Paraffin (5 µg/ml)
  • Western blot (1:32000) & (1 - 3 µg/ml)
  • ELISA (1:32000)

Specificity and Use

TRIM1 / MID2 antibody was raised against synthetic peptide QESPYVSGMKTCH from the C-terminus of human MID2 (NP_036348.2; NP_438112.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset (100%); Mouse, Rat, Dog, Bat, Elephant, Panda, Horse, Pig, Opossum, Turkey, Chicken (92%); Hamster, Rabbit, Zebrafish, Stickleback (85%).
LS-E25491 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B2277. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Human MID2. This antibody is expected to recognise both human isoforms of this protein.
Immunohistochemistry: LS-B2277 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2277 was determined to be 5 ug/ml.


Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Store at -20°C. Avoid freeze-thaw cycles.
For research use only.

About TRIM1 / MID2

Q9UJV3 NM_012216 NP_036348.2

MID2 Antibody, FXY2 Antibody, Midin-2 Antibody, Midline-2 Antibody, Midin 2 Antibody, Midline 2 Antibody, Midline defect 2 Antibody, RNF60 Antibody, RING finger protein 60 Antibody, TRIM1 Antibody, Tripartite motif protein 1 Antibody

TRIM1 / MID2 is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to microtubular structures in the cytoplasm. Alternate splicing of this gene results in two transcript variants encoding different isoforms.


Anti-MID2 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2277 concentration 5 ug/ml.

Western blot

Western blot
Antibody (1 ug/ml) staining of mouse heart lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.

Requested From: 
Date Requested: 2/24/2018

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