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Anti-TPRKB Antibody (clone 3E3) LS-C175151

Catalog Size Price
LS-C175151-100 100 µl (1 mg/ml) Unavailable

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Anti-TPRKB Antibody (clone 3E3) LS-C175151
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IHC - Paraffin, Western blot, Flow Cytometry
Unconjugated
Immunohistochemistry Image
Anti-TPRKB Antibody (clone 3H3) LS-C175160
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Anti-TPRKB Antibody (aa1-175) LS-C185835
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Anti-TPRKB Antibody (clone 1E9) LS-C336942
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Anti-TPRKB Antibody (clone 2165C3a) LS-C342748
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Immunofluorescence Image

100% Guaranteed
Mouse Monoclonal [clone 3E3] (IgG2a) to Human TPRKB
Human
IHC - Paraffin, Western blot, Flow Cytometry
Unconjugated

Details

Human TPRKB
Mouse
Human (tested or 100% immunogen sequence identity)
IgG2a Monoclonal [3E3]
Unconjugated
Protein A/G purified
Unmodified

Applications

  • IHC - Paraffin (1:150)
  • Western blot (1:2000)
  • Flow Cytometry (1:100)

Specificity and Use

TPRKB antibody was raised against full length human recombinant protein of human TPRKB (NP_057142) produced in E. coli.
Human TPRKB

Packaging

PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Store at -20°C. Avoid freeze-thaw cycles.
For research use only.

About TPRKB

Q9Y3C4 NM_016058 NP_057142.1

TPRKB Antibody, CGI-121 Antibody, TP53RK binding protein Antibody, PRPK-binding protein Antibody, TP53RK-binding protein Antibody

Component of the EKC/KEOPS complex that is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. The complex is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. TPRKB acts as an allosteric effector that regulates the t6A activity of the complex. TPRKB is not required for tRNA modification.

Immunohistochemistry

Immunohistochemistry
IHC of paraffin-embedded Carcinoma of Human bladder tissue using anti-TPRKB mouse monoclonal antibody.

Immunohistochemistry

Immunohistochemistry
IHC of paraffin-embedded Human pancreas tissue using anti-TPRKB mouse monoclonal antibody.

Western blot

Western blot
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TPRKB (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TPRKB.

Flow Cytometry

Flow Cytometry
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-TPRKB antibody, and then analyzed by flow cytometry.

Requested From: 
Date Requested: 8/18/2017

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