Human TP73 / p73

Rabbit

Human, Monkey, Mouse, Rat, Bovine, Dog, Hamster (tested or 100% immunogen sequence identity)

Chicken, Xenopus (at least 90% immunogen sequence identity)

Polyclonal

Unconjugated

Immunoaffinity purified

Unmodified

A synthetic peptide C-THSPYAQPSS corresponding to amino acids 95-104 of human p73, GenBank EAW71468.1. This peptide sequence is 100% conserved across multiple p73 isoforms and between rat, mouse, dog and bovine p73. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine (100%); Opossum, Chicken, Xenopus (90%); Bat, Rabbit, Turkey, Stickleback, Medaka, Pufferfish, Zebrafish, Nectri, Fusarium (80%).

aa75-125

This peptide sequence is 100% conserved across multiple p73 isoforms and between rat, mouse, dog and bovine p73. Researchers are encourage to use the the immunogen sequence information and available Bioinformatics data bases to obtain additional information regarding the amino acid sizes associated with various p73 isoforms.

• IHC
• IHC - Paraffin (5 µg/ml)
• Western blot (1 - 3 µg/ml)

Immunohistochemistry: LS-B2084 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2084 was determined to be 5 ug/ml.

PBS, 0.05% Sodium Azide, 0.2% Gelatin

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: O15350 NCBI: NM_005427 NP_005418.1