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Catalog Number Size Price
LS-B2086-50 50 µg (1 mg/ml) $460 
TP73 / p73 Antibody - Anti-p73 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
TP73 / p73 Antibody - Western blot of p73 in A) transfected?cell lysate and B) HeLa cell lysate using antibody at 2 ug/ml.
TP73 / p73 Antibody - Anti-p73 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
TP73 / p73 Antibody - Western blot of p73 in A) transfected?cell lysate and B) HeLa cell lysate using antibody at 2 ug/ml.
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IHC‑plus™ Monoclonal Mouse anti‑Human TP73 / p73 Antibody (clone 5B429, N‑Terminus, IHC, WB) LS‑B2086

IHC‑plus™ Monoclonal Mouse anti‑Human TP73 / p73 Antibody (clone 5B429, N‑Terminus, IHC, WB) LS‑B2086

Note: This antibody replaces LS-C576, LS-C17152
Antibody:
TP73 / p73 Mouse anti-Human Monoclonal (N-Terminus) (5B429) Antibody
Application:
IHC, IHC-P, ICC, WB, IP
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$460
LS-B2086-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
TP73 / p73 Mouse anti-Human Monoclonal (N-Terminus) (5B429) Antibody
Application:
IHC, IHC-P, ICC, WB, IP
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
P73 antibody LS-B2086 is an unconjugated mouse monoclonal antibody to p73 (TP73) (N-Terminus) from human. It is reactive with human and mouse. Validated for ICC, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human TP73 / p73
Synonyms
TP73 | p53-like transcription factor | p73 | p53-related protein | Tumor protein p73
Host
Mouse
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Monoclonal
Clone
5B429
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Immunogen
Full-length human p73. It reacts with an epitope that is located on the N-Terminus of human p73. It does not cross react with p53.
Epitope
N-Terminus
Specificity
Recognizes all of the TAp73 isoforms i. e. isoforms with an intact NH2-terminal transactivation domain (Thottassery et el, 2006, PMID 16505115). Sayan et al 2005 (PMID 15781249) showed that p73 IMG-246/ NBP2-24737 reacted with all tested TAp73 isoforms but not deltaNp73 whereas p73 antibody clone 5B1288 (NB100-56674 / IMG-259A ) showed reactivity against all p73 isoforms including delta Np73. Caspase-cleaved p73 fragments, including 55 kD and 45 kD, have been described in literature (Sayan et al, 2008). p73 antibody clone 5B429 has also been referred to as 429 in the literature.
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • ICC
  • Western blot (2 µg/ml)
  • Immunoprecipitation
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: LS-B2086 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2086 was determined to be 5 ug/ml.
Presentation
PBS, 0.05% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About TP73 / p73
O15350 NM_005427 NP_005418.1

Validation

TP73 / p73 Antibody - Anti-p73 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-p73 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-p73 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

LSBio Ratings

IHC-plus™ TP73 / p73 Antibody (N-Terminus, clone 5B429) for IHC, ICC, WB/Western, IP LS-B2086 has an LSBio Rating of
Laboratory Validation Score (4)

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 8/18/2022