LSBio The Immunohistochemistry Antibody Company
Products
  • Antibodies
  • All Antibodies
  • Primary Antibodies
  • Secondary Antibodies
  • IHC-plus Antibodies
  • Isotype Control Antibodies
  • ELISA Kits
  • All Kits
  • Sandwich ELISA Kits
  • Competitive EIA Kits
  • Cell-Based ELISA Kits
  • DNA-Binding ELISA Kits
  • Direct ELISA Kits
  • Functional ELISA Kits
  • Phospho-Specific ELISA Kits
  • Custom ELISA Kits
  • Proteins
  • All Proteins
  • Recombinant Proteins
  • Native Proteins
  • Over-expression Lysates
  • Cell and Tissue Lysates
  • Bio-active Proteins
  • Animal-free Proteins
  • Synthetic Peptides
  • Immunohistochemistry
  • Comprehensive IHC Reports
  • Custom IHC Services
  • TCR Screening Services
  • IHC-plus Antibodies
  • Other
  • Blocking Peptides
Services
  • Immunohistochemistry Services

  • Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

  • TCR Screening Services

  • Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Have a question? CustomerSupport@LSBio.com
Resources
  • Purchasing
  • Product Ordering Terms and Conditions
  • Holiday Schedule
  • About the LSBio Guarantee
  • About Our Rewards Program
  • Reference Material
  • About LSBio (LifeSpan BioSciences Inc.)
  • About Our 2-million Specimen Tissue Bank
  • About Our IHC Validation Process
  • About Our IHC-plus™ Immunohistochemistry Protocol
  • Publications
  • Secure Logins
  • Login to Our Secure FTP Site
  • Login to Our IHC Database
Distributors
Contact Us

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)
How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Quick Order ▾
View Cart

Anti-TNFSF11 / RANKL / TRANCE Antibody (aa143-316) LS-C154136

Ordering

Wt. Vol. Conc. Price
100 µg 100 µl - $405
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular TNFSF11 / RANKL / TRANCE Antibodies

Anti-TNFSF11 / RANKL / TRANCE Antibody (Internal) IHC-plus™ LS-B1425
Rabbit Polyclonal to Human TNFSF11 / RANKL / TRANCE
Human, Mouse, Rat
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-TNFSF11 / RANKL / TRANCE Antibody (Biotin) LS-C86684
Goat Polyclonal to Human TNFSF11 / RANKL / TRANCE
Human
Western blot, ELISA
Biotin Conjugated

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Mouse TNFSF11 / RANKL / TRANCE
Mouse, Rat
Western blot, ELISA
Unconjugated

Details

Mouse TNFSF11 / RANKL / TRANCE
Rabbit
Mouse, Rat (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Ion exchange chromatography
Unmodified

Applications

  • Western blot (1:1000)
  • ELISA

Specificity and Use

TNFSF11 / RANKL / TRANCE antibody was raised against this IgG fraction antibody was prepared from rabbit antiserum after repeated immunizations with recombinant truncated mouse RANKL protein (aa 143-316) produced in E. coli.
aa143-316
This antibody is specific for mouse RANKL protein. A BLAST analysis was used to suggest cross-reactivity with RANKL from mouse sources based on 100% homology with the immunizing sequence. Based on 97% homology, cross-reactivity with rat, 91% with Chinese hamster, 90% with Thirteen-lined ground squirrel; other sources has not been determined.
This purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 19-20 kD in size corresponding to the mature mouse RANKL protein by western blotting in appropriate cell lysate or extract.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2
100 µl deionized water
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

About TNFSF11 / RANKL / TRANCE

O14788 AF053712 AAC39731.1

TNFSF11 Antibody, CD254 Antibody, CD254 antigen Antibody, HRANKL2 Antibody, OPTB2 Antibody, TRANCE Antibody, ODF Antibody, OPGL Antibody, SOdf Antibody, Osteoprotegerin ligand Antibody, RANKL Antibody

TNFSF11 / RANKL / TRANCE is a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss.

Western blot

Western blot
Anti-Mouse RANKL Antibody - Western Blot. Anti-mouse RANKL antibody in western blot shows detection of recombinant mouse RANKL raised in E. coli. Recombinant truncated protein (0.1 ug, 19.9 kD) was loaded on to an SDS-PAGE gel, and after separation, transferred to nitrocellulose. The membrane was blocked with 1% BSA in TBST for 30 min at RT, followed by incubation with Anti-Mouse RANKL antibody diluted 1:1000 in 1% BSA in TBST overnight at 4°C. After washes, the blot was reacted with secondary antibody Dylight 649 Conjugated Anti-Rabbit IgG (H&L) (Goat) Antibody ( diluted 1:20000 in blocking buffer (MB-070) for 30 min. at RT. Data was collected using Bio-Rad VersaDoc 4000 MP imaging system.
 

Requested From: United States
Date Requested: 12/6/2016

Get Social With Us! Follow us on Facebook Follow us on Google+ Follow us on LinkedIn
Copyright © 2016 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy
Catalog Number