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| Catalog Number | Size | Price |
|---|---|---|
| LS-C154136-100 | 100 µg (1 mg/ml) | $567 |
Polyclonal Rabbit anti‑Mouse TNFSF11 / RANKL / TRANCE Antibody (WB) LS‑C154136
Polyclonal Rabbit anti‑Mouse TNFSF11 / RANKL / TRANCE Antibody (WB) LS‑C154136
Antibody:
TNFSF11 / RANKL / TRANCE Rabbit anti-Mouse Polyclonal Antibody
Application:
WB, ELISA
Reactivity:
Mouse, Rat
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
TNFSF11 / RANKL / TRANCE Rabbit anti-Mouse Polyclonal Antibody
Application:
WB, ELISA
Reactivity:
Mouse, Rat
Format:
Unconjugated, Unmodified
Specifications
Description
TRANCE antibody LS-C154136 is an unconjugated rabbit polyclonal antibody to TRANCE (TNFSF11 / RANKL) from mouse. It is reactive with mouse and rat. Validated for ELISA and WB.
Target
Mouse TNFSF11 / RANKL / TRANCE
Synonyms
TNFSF11 | CD254 | CD254 antigen | HRANKL2 | OPTB2 | TRANCE | ODF | OPGL | SOdf | Osteoprotegerin ligand | RANKL
Host
Rabbit
Reactivity
Mouse, Rat
(tested or 100% immunogen sequence identity)
Predicted
Rat, Ground squirrel, Hamster-Chinese (at least 90% immunogen sequence identity)
Clonality
IgG
Polyclonal
Conjugations
Unconjugated
Purification
Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
Modifications
Unmodified
Immunogen
This IgG fraction antibody was prepared from rabbit antiserum after repeated immunizations with recombinant truncated mouse RANKL protein (aa 143-316) produced in E.coli.
Specificity
This antibody is specific for mouse RANKL protein. A BLAST analysis was used to suggest cross-reactivity with RANKL from mouse sources based on 100% homology with the immunizing sequence. Based on 97% homology, cross-reactivity with rat, 91% with Chinese hamster, 90% with Thirteen-lined ground squirrel; other sources has not been determined.
Applications
- Western blot (1:1000)
- ELISA (1:10000)
Usage
This purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 19-20 kD in size corresponding to the mature mouse RANKL protein by western blotting in appropriate cell lysate or extract.
Presentation
Lyophilized from 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl
Reconstitution
Reconstitute in 100 µL of deionized water.
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About TNFSF11 / RANKL / TRANCE
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Featured Products
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, Western blot, ELISA
Species:
Human
Applications:
Western blot, ELISA
Species:
Mouse, Human
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human
Applications:
Western blot, ELISA
Species:
Human
Applications:
Western blot
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/25/2024
Date Requested: 4/25/2024
