Target
Human TMEM142A / ORAI1
Synonyms
ORAI1 | CRACM1 | ORAT1 | Protein orai-1 | TMEM142A | Transmembrane protein 142A
Reactivity
Human, Dog, Pig, Rabbit
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence C-KKQPGQPRPTSKP, from the internal region of the protein sequence according to NP_116179.2.
Applications
- Western blot (0.03 - 0.1 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:64000)
Usage
Peptide ELISA: antibody detection limit dilution 1:64000. Western Blot: Approx. 50-55kD band observed in Human Liver and Human Placenta lysates (calculated MW of 32.7kD according to NP_116179.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Li et al,, J Biol Chem. 2007 Oct 5;282(40):29448-56.; PMID: 17702753). Recommended concentration: 0.03-0.1 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee