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Anti-TMEM142A / ORAI1 Antibody (aa203-215) LS-C87376

Catalog	Size	Price
LS-C87376-100	100 µg (0.5 mg/ml)	Unavailable

Related Products

45 TMEM142A / ORAI1 Antibodies

Most Popular TMEM142A / ORAI1 Antibodies

Anti-TMEM142A / ORAI1 Antibody (C-Terminus) LS-C34723

Antibody:	Rabbit Polyclonal (IgG) to Human TMEM142A / ORAI1
Reactivity:	Human, Mouse, Rat
Application:	IHC, Western blot
Format:	Unconjugated

Immunohistochemistry of ORAI1 in human spleen tissue with ORAI1 antibody at 2.5 ug/ml.

Anti-TMEM142A / ORAI1 Antibody (N-Terminus) LS-C81517

Antibody:	Rabbit Polyclonal (IgG) to Human TMEM142A / ORAI1
Reactivity:	Human
Application:	Western blot
Format:	Unconjugated

HepG2 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Anti-TMEM142A / ORAI1 Antibody (aa203-215) LS-C87376

Antibody:	Goat Polyclonal to Human TMEM142A / ORAI1
Reactivity:	Human, Dog, Pig, Rabbit
Application:	Western blot, ELISA
Format:	Unconjugated

Antibody (0.03 ug/ml) staining of Human Placenta lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence

Anti-TMEM142A / ORAI1 Antibody (C-Terminus, clone 6D11A11) LS-C144492

Antibody:	Mouse Monoclonal [clone 6D11A11] to Human TMEM142A / ORAI1
Reactivity:	Human
Application:	IHC, Western blot
Format:	Unconjugated

Immunohistochemistry of ORAI1 in human spleen tissue with ORAI1 antibody at 5 ug/mL.

Anti-TMEM142A / ORAI1 Antibody (aa145-173) LS-C163265

Antibody:	Rabbit Polyclonal to Human TMEM142A / ORAI1
Reactivity:	Human, Mouse
Application:	IHC, IHC - Paraffin, Western blot, ELISA
Format:	Unconjugated

Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with ORAI1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Antibody:	Goat Polyclonal to Human TMEM142A / ORAI1
Reactivity:	Human, Dog, Pig, Rabbit
Application:	Western blot, ELISA
Format:	Unconjugated

Details

Target:	Human TMEM142A / ORAI1
Host:	Goat
Reactivity:	Human, Dog, Pig, Rabbit (tested or 100% immunogen sequence identity)
Predicted:	Monkey, Bat, Bovine, Horse (at least 90% immunogen sequence identity)
Clonality:	Polyclonal
Conjugations:	Unconjugated
Purification:	Immunoaffinity purified
Modifications:	Unmodified

Applications

Approved:

Western blot (0.03 - 0.1 µg/ml)
ELISA (1:64000) & (0.03 - 0.1 µg/ml)

Failed:

IHC - Paraffin

Specificity and Use

Immunogen:

Synthetic peptide C-KKQPGQPRPTSKP from an internal region of human ORAI1 (NP_116179.2). Percent identity by BLAST analysis: Human, Gorilla, Monkey, Panda, Dog, Rabbit, Pig (100%); Marmoset, Bovine, Bat, Horse (92%); Elephant (85%).

Blocking Peptide:

LS-E26918 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C87376. Useful for pre-absorption and neutralization of the antibody's antigen binding site.

Epitope:

aa203-215

Specificity:

Human ORAI1.

Usage:

Peptide ELISA: antibody detection limit dilution 1:64000. Western Blot: Approx. 50-55kD band observed in Human Liver and Human Placenta lysates (calculated MW of 32.7kD according to NP_116179.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Li et al,, J Biol Chem. 2007 Oct 5;282(40):29448-56.; PMID: 17702753). Recommended concentration: 0.03-0.1 ug/ml.

Packaging

Presentation:	Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Storage:	Store at -20°C. Avoid freeze-thaw cycles.
Restrictions:	For research use only.

About TMEM142A / ORAI1

Uniprot: Q96D31 NCBI: NM_032790 NP_116179.2

Synonyms: ORAI1 Antibody, CRACM1 Antibody, ORAT1 Antibody, Protein orai-1 Antibody, TMEM142A Antibody, Transmembrane protein 142A Antibody

Description: Ca2+ release-activated Ca2+ (CRAC) channel subunit which mediates Ca2+ influx following depletion of intracellular Ca2+ stores and channel activation by the Ca2+ sensor, STIM1. CRAC channels are the main pathway for Ca2+ influx in T-cells and promote the immune response to pathogens by activating the transcription factor NFAT.

(More About TMEM142A / ORAI1)

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Western blot

Antibody (0.03 ug/ml) staining of Human Placenta lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence

Requested From:
Date Requested: 4/24/2018

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