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Anti-TLK1 Antibody (aa737-751) LS-C18924

Ordering

Wt. Vol. Conc. Price
100 µg - - Unavailable

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60 TLK1 Antibodies

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Anti-TLK1 Antibody (aa720-750) LS-C101204
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Anti-TLK1 Antibody (clone 4B3) LS-C133607
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Anti-TLK1 Antibody (aa730-779) IHC-plus™ LS-B9860
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Anti-TLK1 Antibody LS-C410446
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100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human TLK1
Human, Dog
Western blot, ELISA
Unconjugated

Details

Human TLK1
Rabbit
Human, Dog (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • Western blot (1:200 - 1:2000)
  • ELISA (1:10000 - 1:50000)

Specificity and Use

TLK1 antibody was raised against synthetic peptide from human TLK1.
aa737-751
Amino acids 737-751 of human Tlk1 (Tousled-like kinase) (isoform 1) protein.
This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 86 kD, 89 kD or 64 kD in size corresponding to isoforms of TLk-1 by western blotting in the appropriate cell lysate or extract. This antibody reacts with both phosphorylated and non-phosphorylated forms of Tlk-1 at the S743 residue (isoform 1) of the protein.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Store vial at -20 C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20 C or below. Avoid cycles of freezing and thawing.
For research use only.

About TLK1

Q9UKI8 NM_012290 NP_036422.3

TLK1 Antibody, KIAA0137 Antibody, Tousled-like kinase 1 Antibody, Snare kinase snak Antibody, PKU-beta Antibody, SNARE protein kinase SNAK Antibody

Rapidly and transiently inhibited by phosphorylation following the generation of DNA double-stranded breaks during S-phase. This is cell cycle checkpoint and ATM-pathway dependent and appears to regulate processes involved in chromatin assembly. Isoform 3 phosphorylates and enhances the stability of the t-SNARE SNAP23, augmenting its assembly with syntaxin. Isoform 3 protects the cells from the ionizing radiation by facilitating the repair of DSBs.

Western blot

Western blot
Anti-Tlk-1 Antibody - Western Blot. Western blot of Affinity Purified anti-Tlk-1 antibody shows detection of a band ~64 kD corresponding to isoform 3 of human Tlk-1 (arrowhead). Specific reactivity with this band is blocked when the antibody is pre-incubated with the immunizing peptide (data not shown). Approximately 30 ug of a HeLa nuclear extract was separated by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:250 for 2h at room temperature followed by washes and reaction with a 1:10000 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG [H&L] MX ( for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

Requested From: 
Date Requested: 4/23/2017

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