Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Thyroid hormone receptor alpha (TR alpha), a NR1 Thyroid Hormone-Like Receptor, mediates the diverse actions of thyroid hormone in development, differentiation, and metabolism. Four protein isoforms are encoded by the same TR alpha genomic locus: TR alpha1 (410 aa) (Nakai et al. 1988), TR alpha2 (490 aa) ( Nakai et al. 1988), TR deltaalpha1 (~154 aa), and TR deltaalpha2 (~237 aa); however, only TR alpha1 is able to bind the ligand ( Lazar et al. 1988).
Immunofluorescence of paraformaldehyde-fixed HeLa using Thyroid Hormone Receptor alpha 2 antibody at 1:500 dilution.
Sample (30 ug of whole cell lysate). A: JurKat. 10% SDS PAGE. THRA antibody diluted at 1:1000.
Thyroid Hormone Receptor alpha antibody immunoprecipitates Thyroid Hormone Receptor alpha protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. Control with 2 ug of preimmune rabbit IgG B. Immunoprecipitation of Thyroid Hormone Receptor alpha protein by 2 ug of Thyroid Hormone Receptor alpha antibody 7.5% SDS-PAGE The immunoprecipitated Thyroid Hormone Receptor alpha protein was detected by Thyroid Hormone Receptor alpha antibody diluted at 1:1000. EasyBlot anti-rabbit IgG (anti-rabbit IgG (HRP) -01) was used as a secondary reagent.
Requested From: United States
Date Requested: 2/28/2017