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Catalog Number Size Price
LS-C60119-100 100 µg (1 mg/ml) $567 
THRA / THR Alpha Antibody - Anti-THRA Antibody - Western Blot. Western blot of affinity purified anti-THRA antibody shows detection of purified recombinant THRA (lane 1) and THRA present in a 293 cell lysate after transient transfection with THRA (lane 3). No staining is evident in lysates from mock-transfected 293 cells (lane 2). Endogenous THRA is not detected in mouse brain whole cell lysate (lane 4). Nuclear extracts may be required to detect endogenous THRA as the protein localizes within the nucleus. The band at ~55 kD, indicated by the arrowhead, corresponds to THRA. Personal communication, S. Cheng and H. Ying, NCI, Bethesda, MD.

Polyclonal Rabbit anti‑Human THRA / THR Alpha Antibody (IHC, IF, WB) LS‑C60119

Polyclonal Rabbit anti‑Human THRA / THR Alpha Antibody (IHC, IF, WB) LS‑C60119

Antibody:
THRA / THR Alpha Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IF, WB, IP, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$567
LS-C60119-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
THRA / THR Alpha Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IF, WB, IP, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
THR Alpha antibody LS-C60119 is an unconjugated rabbit polyclonal antibody to human THR Alpha (THRA). Validated for ELISA, IF, IHC, IP and WB. Cited in 1 publication.
Target
Human THRA / THR Alpha
Synonyms
THRA | AR7 | C-ERBA-1 | C-erbaalpha | EAR-7.1/EAR-7.2 | ERB-T-1 | ERBA1 | EAR-7 | ERBA-related 7 | HTR alpha 1 | HTR alpha 2 | THRA1 | Thyroid hormone receptor alpha | T3R | Tralpha2 | Triiodothyronine receptor | V-erbA-related protein 7 | Thyroid hormone receptor | Tralpha1 | NR1A1 | THRA3 | C-erbA-alpha | CHNG6 | EAR7 | ERBA | Erba-alpha | THRA2 | TR alpha | Tralpha
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the amino terminus of human THRA isoform 1 protein.
Specificity
This antibody reacts with human THRA protein. A BLAST analysis was used to suggest cross-reactivity with THRA from mouse, human and rat based on a 100% homology with the immunizing sequence. Cross-reactivity with THRA from other sources has not been determined.
Applications
  • IHC (1:200)
  • Immunofluorescence (1:200)
  • Western blot (1:500 - 1:2000)
  • Immunoprecipitation (1:100)
  • ELISA (1:300000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~55 kD in size corresponding to THRA by western blotting in the appropriate cell lysate or extract. This antibody is capable of detecting transiently transfected THRA. Detection of endogenous THRA must be optimized by the end user.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: -20°C; Long term: -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About THRA / THR Alpha
P10827 NM_003250 NP_003241.2

LSBio Ratings

THRA / THR Alpha Antibody for IHC, IF/Immunofluorescence, WB/Western, IP, ELISA LS-C60119 has an LSBio Rating of
Publications (4)

Publications (1)

Persistent COUP-TFII expression underlies the myopathy and impaired muscle regeneration observed in resistance to thyroid hormone-alpha. Paola Aguiari, Yan-Yun Liu, Astgik Petrosyan, Sheue-Yann Cheng, Gregory A Brent, Laura Perin, Anna Milanesi. Scientific reports. 2021 February;11:4601.

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/25/2024