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Anti-TGFB4 / LEFTY2 Antibody (clone 7C5G1H6H10) IHC-plus™ LS-B2540

Note: This antibody replaces LS-C18793

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
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Orders@LSBio.com
 

Most Popular TGFB4 / LEFTY2 Antibodies

Anti-TGFB4 / LEFTY2 Antibody (clone 7C5G1H6H10) IHC-plus™ LS-B2540
Mouse Monoclonal [clone 7C5G1H6H10] (IgG2a,k) to Human TGFB4 / LEFTY2
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-TGFB4 / LEFTY2 Antibody (N-Terminus) IHC-plus™ LS-B6826
Rabbit Polyclonal (IgG) to Human TGFB4 / LEFTY2
Human, Rat, Pig
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-TGFB4 / LEFTY2 Antibody (aa247-358, FITC) LS-C303875
Rabbit Polyclonal (IgG) to Mouse TGFB4 / LEFTY2
Mouse
Western blot, ELISA
FITC Conjugated
Western blot Image

100% Guaranteed 100% Guaranteed Publications Publications (2)
Mouse Monoclonal [clone 7C5G1H6H10] (IgG2a,k) to Human TGFB4 / LEFTY2
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human TGFB4 / LEFTY2
Mouse
Human (tested or 100% immunogen sequence identity)
IgG2a,k Monoclonal [7C5G1H6H10]
Unconjugated
Protein A purified
Unmodified

Applications

  • IHC - Paraffin (10 µg/ml)
  • Western blot
  • ELISA

Specificity and Use

TGFB4 / LEFTY2 antibody was raised against recombinant human LEFTY2.
A BALB/c mouse was immunized with a recombinant form of 6X HIS tagged human LEFTY. Hybridoma: Produced by the fusion between BALB/c mouse splenocytes and mouse Sp2/0 myeloma cells using conventional hybridoma technology.
Immunohistochemistry: LS-B2540 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2540 was determined to be 10 ug/ml.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide. Sourced in TCS.
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

Publications (2)

1
Multiple left-right asymmetry defects in Shh(-/-) mutant mice unveil a convergence of the shh and retinoic acid pathways in the control of Lefty-1. Tsukui T, Capdevila J, Tamura K, Ruiz-Lozano P, Rodriguez-Esteban C, Yonei-Tamura S, Magalln J, Chandraratna RA, Chien K, Blumberg B, Evans RM, Belmonte JC. Proceedings of the National Academy of Sciences of the United States of America. 1999 96:11376-81. [PubMed:10500184] [PMC:PMC18041]
2
Lefty inhibits receptor-regulated Smad phosphorylation induced by the activated transforming growth factor-beta receptor. Ulloa L, Tabibzadeh S. The Journal of biological chemistry. 2001 276:21397-404. [PubMed:11278746]

About TGFB4 / LEFTY2

O00292 NM_003240 NP_003231.2

LEFTY2 Antibody, EBAF Antibody, LEFTYA Antibody, Protein lefty-2 Antibody, LEFTA Antibody, TGFB4 Antibody, Protein lefty-A Antibody, TGF-beta-4 Antibody

TGFB4 / LEFTY2 is a member of the TGF-beta family of proteins. The encoded protein is secreted and plays a role in left-right asymmetry determination of organ systems during development. The protein may also play a role in endometrial bleeding. Mutations in this gene have been associated with left-right axis malformations, particularly in the heart and lungs. Some types of infertility have been associated with dysregulated expression of this gene in the endometrium.

Immunohistochemistry

Immunohistochemistry
Anti-LEFTY2 antibody IHC of human uterus, myometrium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2540 concentration 10 ug/ml.

Western blot

Western blot
Mab anti-Human LEFTY antibody (clone 7C5G1H6H10) is shown to detect by western blot partially purified recombinant 6X His tagged human LEFTY. Detection occurs after 1.0 ug of protein is loaded in each lane. The blot was incubated with a 1:2000 dilution of Mab anti-Human LEFTY at room temperature for 30 min followed by detection using IRDye800 labeled Goat-a-Mouse IgG [H&L] (610-132-121) diluted 1:1000. Lane 1 contains an unrelated 6X His tagged protein and shows that the antibody does not recognize the epitope tag. Lane 2 contains partially purified recombinant human LEFTY. The antibody may be used to detect endogenous human LEFTY. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

Western blot

Western blot
Mab anti-Human LEFTY antibody (clone 7C5G1H6H10) is shown to detect by western blot partially purified recombinant 6X His tagged human LEFTY. 1.0 mg of protein of protein was loaded per lane. The blot was incubated with a 1:2000 dilution of Mab anti-Human LEFTY.
 

Requested From: United States
Date Requested: 12/6/2016

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