Human TGFB4 / LEFTY2
(tested or 100% immunogen sequence identity)
Protein A purified
- IHC - Paraffin (10 µg/ml)
- Western blot
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TGFB4 / LEFTY2 antibody was raised against recombinant human LEFTY2.
A BALB/c mouse was immunized with a recombinant form of 6X HIS tagged human LEFTY. Hybridoma: Produced by the fusion between BALB/c mouse splenocytes and mouse Sp2/0 myeloma cells using conventional hybridoma technology.
Immunohistochemistry: LS-B2540 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2540 was determined to be 10 ug/ml.
0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide. Sourced in TCS.
4°C or -20°C, Avoid freeze-thaw cycles.
For research use only.
This antibody carries the LSBio 100% Guarantee.