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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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TFB1M Antibody (aa36‑85) LS‑C109926
TFB1M antibody LS-C109926 is an unconjugated rabbit polyclonal antibody to TFB1M from human, bovine, dog and other species. Validated for WB.
Catalog
Size
Price
LS-C109926-50
50 µg
Unavailable

Popular TFB1M Products

TFB1M antibody (0.3 ug/ml) staining of A431 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Species: Human
Applications: Western blot, Peptide Enzyme-Linked Immunosorbent Assay
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TFB1M (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TFB1M.
Species: Human
Applications: Western blot
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TFB1M (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TFB1M.
Species: Human
Applications: Western blot
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TFB1M (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TFB1M.
Species: Human
Applications: Western blot
Immunohistochemistry image at a dilution of 1:500 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 °C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Species: Human
Applications:

Product Description

TFB1M antibody LS-C109926 is an unconjugated rabbit polyclonal antibody to TFB1M from human, bovine, dog and other species. Validated for WB.
About TFB1M
S-adenosyl-L-methionine-dependent methyltransferase which specifically dimethylates mitochondrial 12S rRNA at the conserved stem loop. Also required for basal transcription of mitochondrial DNA, probably via its interaction with POLRMT and TFAM. Stimulates transcription independently of the methyltransferase activity. Q8WVM0 NM_016020 NP_057104.2

TFB1M Antibody, CGI-75 Antibody, H-mtTFB Antibody, H-mtTFB1 Antibody, HTFB1M Antibody, MtTFB1 Antibody, MtTFB Antibody, CGI75 Antibody


Specifications

Target
Human TFB1M
Host
Rabbit
Reactivity
Human, Bovine, Dog, Guinea pig, Zebrafish (tested or 100% immunogen sequence identity)
Predicted
Monkey, Horse, Pig, Rabbit, Xenopus (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
TFB1M antibody was raised against synthetic peptide located between aa36-85 of human TFB1M (Q8WVM0, NP_057104). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Galago, Dog, Bovine, Guinea pig (100%); Monkey, Marmoset, Elephant, Rabbit, Horse, Pig, Xenopus (92%); Bat (85%); Zebrafish (84%).
Blocking Peptide
LS-E12258 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C109926. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa36-85
Specificity
Human TFB1M
Usage
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

TFB1M antibody LS-C109926 Western blot of Fetal liver lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
TFB1M antibody LS-C109926 Western blot of Fetal liver lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

TFB1M antibody LS-C109926 Western blot of Fetal liver lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
TFB1M antibody LS-C109926 Western blot of Fetal liver lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

TFB1M antibody LS-C109926 Western blot of Fetal liver lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
TFB1M antibody LS-C109926 Western blot of Fetal liver lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

TFB1M antibody LS-C109926 Western blot of Fetal liver lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
TFB1M antibody LS-C109926 Western blot of Fetal liver lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/25/2018

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