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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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TFAP2A / AP‑2 Antibody (C‑Terminus) LS‑C31044
AP-2 antibody LS-C31044 is an unconjugated rabbit polyclonal antibody to AP-2 (TFAP2A) from human, bovine and monkey. Validated for WB.
Catalog
Size
Price
LS-C31044-100
100 µg (1 mg/ml)
Unavailable

Popular TFAP2A / AP-2 Products

Western blot of AP-2 in MCF-7 cell lysate with antibody. A protein band of an approximate molecular weight of 55 kD is detected.
Species: Human, Monkey, Mouse, Rat, Bovine, Dog, Sheep, Xenopus
Applications: Western blot
Anti-TFAP2A / AP2 Alpha antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B427 concentration 5 ug/ml.
Species: Human, Mouse, Rat, Dog, Chicken
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Antibody
Species: Zebrafish
Applications: Peptide Enzyme-Linked Immunosorbent Assay
Anti-TFAP2A / AP2 Alpha antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6155 concentration 5 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Immunofluorescence analysis of Hela cells using TFAP2A mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher
Species: Human
Applications: ICC, Western blot, Flow Cytometry, ELISA

Product Description

AP-2 antibody LS-C31044 is an unconjugated rabbit polyclonal antibody to AP-2 (TFAP2A) from human, bovine and monkey. Validated for WB.
About TFAP2A / AP-2
Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. P05549 NM_003220 NP_003211.1

TFAP2A Antibody, Activator protein 2 Antibody, AP-2 Antibody, AP-2a Antibody, AP2TF Antibody, AP-2alpha Antibody, BOFS Antibody, TFAP2 Antibody, Transcription factor AP-2 Antibody, AP-2 transcription factor Antibody, AP2-alpha Antibody


Specifications

Target
Human TFAP2A / AP-2
Host
Rabbit
Reactivity
Human, Monkey, Bovine (tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat, Bat, Dog, Guinea pig, Horse, Pig, Rabbit, Sheep (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Protein A purified
Modifications
Unmodified
Applications
  • Western blot (1.25 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
TFAP2A / AP-2 antibody was raised against synthetic peptide from C-Terminus of human TFAP2A (P05549, NP_003211). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Elephant, Bovine (100%); Galago, Mouse, Rat, Sheep, Dog, Bat, Rabbit, Horse, Pig, Guinea pig (92%); Opossum, Chicken, Platypus (85%); Turkey (83%).
Blocking Peptide
LS-E10855 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C31044. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
C-Terminus
Specificity
Human TFAP2A / AP2 Alpha
Presentation
Lyophilized.
Reconstitution
100 µl After adding water, will consist of PBS buffer with 2% sucrose
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

A: Marker, B: HepG2 Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
A: Marker, B: HepG2 Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

A: Marker, B: HepG2 Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
A: Marker, B: HepG2 Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

A: Marker, B: HepG2 Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
A: Marker, B: HepG2 Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

A: Marker, B: HepG2 Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
A: Marker, B: HepG2 Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/24/2018

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