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Anti-TFAP2A / AP-2 Antibody (aa3-14) IHC-plus™ LS-B427

Note: This antibody replaces LS-C18899

Ordering

Wt. Vol. Conc. Price
50 µg - - Unavailable

Related Products

61 TFAP2A / AP-2 Antibodies

Most Popular TFAP2A / AP-2 Antibodies

Anti-TFAP2A / AP-2 Antibody (aa100-150) LS-C624
Rabbit Polyclonal (IgG) to Human TFAP2A / AP-2
Human, Monkey, Mouse, Rat, Bovine, Dog, Sheep, Xenopus
Western blot
Unconjugated
Western blot Image
Anti-TFAP2A / AP-2 Antibody (aa3-14) IHC-plus™ LS-B427
Goat Polyclonal to Human TFAP2A / AP-2
Human, Mouse, Rat, Dog, Chicken
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-TFAP2A / AP-2 Antibody (aa405-419) LS-C87212
Goat Polyclonal to Zebrafish TFAP2A / AP-2
Zebrafish
ELISA
Unconjugated
Anti-TFAP2A / AP-2 Antibody (clone 2G5) IHC-plus™ LS-B6155
Mouse Monoclonal [clone 2G5] (IgG1,k) to Human TFAP2A / AP-2
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-TFAP2A / AP-2 Antibody (Lys431) LS-C175920
Rabbit Polyclonal to Human TFAP2A / AP-2
Human, Mouse, Rat
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image

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Goat Polyclonal to Human TFAP2A / AP-2
Human, Mouse, Rat, Dog, Chicken
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated

Details

Human TFAP2A / AP-2
Goat
Human, Mouse, Rat, Dog, Chicken (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (2.5 µg/ml)
  • Immunofluorescence
  • Western blot (1:500 - 1:2000)
  • ELISA (1:5000 - 1:20000)

Specificity and Use

TFAP2A / AP-2 antibody was raised against synthetic peptide from human TFAP2A / AP2 Alpha.
aa3-14
aa 3-14 of Human AP2A1 and AP2A2 proteins.
Immunohistochemistry: LS-B427 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B427 was determined to be 2.5 ug/ml.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

About TFAP2A / AP-2

P05549 NM_003220 NP_003211.1

TFAP2A Antibody, Activator protein 2 Antibody, AP-2 Antibody, AP-2a Antibody, AP2TF Antibody, AP-2alpha Antibody, BOFS Antibody, TFAP2 Antibody, Transcription factor AP-2 Antibody, AP-2 transcription factor Antibody, AP2-alpha Antibody

Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC.

Immunohistochemistry

Immunohistochemistry
Anti-TFAP2A / AP2 Alpha antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B427 concentration 5 ug/ml.

Western blot

Western blot
Anti-AP2A Antibody - Western Blot. Western blot of Affinity Purified anti-AP2A antibody shows detection of a band just below 100 kD corresponding to Human AP2A1 in a various preparations. Lane 1 - HeLa nuclear extract, Lane 2 - HeLa, Lane 3 - 293, Lane 4 - A431 and Lane 5 - Jurkat whole cell lysates. In lanes 6-10 the antibody was preincubated with 1 ug/ml of the immunizing peptide which effectively blocks the specific reactivity of this antibody with AP2A. Approximately 20 ug of each lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-AP2A antibody. Detection occurred using a 1:5000 dilution of HRP-labeled Rabbit anti-Goat IgG for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche) using a 60-sec exposure time.

Requested From: 
Date Requested: 4/24/2017

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