LSBio The Immunohistochemistry Antibody Company
  • Antibodies
  • All Antibodies
  • Primary Antibodies
  • Secondary Antibodies
  • IHC-plus Antibodies
  • Isotype Control Antibodies
  • ELISA Kits
  • All Kits
  • Sandwich ELISA Kits
  • Competitive EIA Kits
  • Cell-Based ELISA Kits
  • DNA-Binding ELISA Kits
  • Direct ELISA Kits
  • Functional ELISA Kits
  • Phospho-Specific ELISA Kits
  • Custom ELISA Kits
  • Proteins
  • All Proteins
  • Recombinant Proteins
  • Native Proteins
  • Over-expression Lysates
  • Cell and Tissue Lysates
  • Bio-active Proteins
  • Animal-free Proteins
  • Synthetic Peptides
  • Immunohistochemistry
  • Comprehensive IHC Reports
  • Custom IHC Services
  • TCR Screening Services
  • IHC-plus Antibodies
  • Other
  • Blocking Peptides
  • Immunohistochemistry Services

  • Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

  • TCR Screening Services

  • Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Have a question?
  • Purchasing
  • Product Ordering Terms and Conditions
  • Holiday Schedule
  • About the LSBio Guarantee
  • About Our Rewards Program
  • Reference Material
  • About LSBio (LifeSpan BioSciences Inc.)
  • About Our 2-million Specimen Tissue Bank
  • About Our IHC Validation Process
  • About Our IHC-plus™ Immunohistochemistry Protocol
  • Publications
  • Secure Logins
  • Login to Our Secure FTP Site
  • Login to Our IHC Database
Contact Us

2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)
How to Buy - Details about how to buy our products. - To submit a new order. - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues. - To request technical information requests about an LSBio product or its application. - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Quick Order ▾
View Cart

Anti-TFAP2A / AP-2 Antibody (aa3-14) IHC-plus™ LS-B427

Note: This antibody replaces LS-C18899


Wt. Vol. Conc. Price
50 µg - - $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)

Most Popular TFAP2A / AP-2 Antibodies

Anti-TFAP2A / AP-2 Antibody (aa100-150) LS-C624
Rabbit Polyclonal (IgG) to Human TFAP2A / AP-2
Human, Monkey, Mouse, Rat, Bovine, Dog, Sheep, Xenopus
Western blot
Western blot Image
Anti-TFAP2A / AP-2 Antibody (aa405-419) LS-C87212
Goat Polyclonal to Zebrafish TFAP2A / AP-2

100% Guaranteed 100% Guaranteed
Goat Polyclonal to Human TFAP2A / AP-2
Human, Mouse, Rat, Dog, Chicken
IHC - Paraffin, Immunofluorescence, Western blot, ELISA


Human TFAP2A / AP-2
Human, Mouse, Rat, Dog, Chicken (tested or 100% immunogen sequence identity)
Immunoaffinity purified


  • IHC - Paraffin (2.5 µg/ml)
  • Immunofluorescence
  • Western blot (1:500 - 1:2000)
  • ELISA (1:5000 - 1:20000)

Specificity and Use

TFAP2A / AP-2 antibody was raised against synthetic peptide from human TFAP2A / AP2 Alpha.
aa 3-14 of Human AP2A1 and AP2A2 proteins.
Immunohistochemistry: LS-B427 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B427 was determined to be 2.5 ug/ml.


0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

About TFAP2A / AP-2

P05549 NM_003220 NP_003211.1

TFAP2A Antibody, Activator protein 2 Antibody, AP-2 Antibody, AP-2a Antibody, AP2TF Antibody, AP-2alpha Antibody, BOFS Antibody, TFAP2 Antibody, Transcription factor AP-2 Antibody, AP-2 transcription factor Antibody, AP2-alpha Antibody

Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC.


Anti-TFAP2A / AP2 Alpha antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B427 concentration 5 ug/ml.

Western blot

Western blot
Anti-AP2A Antibody - Western Blot. Western blot of Affinity Purified anti-AP2A antibody shows detection of a band just below 100 kD corresponding to Human AP2A1 in a various preparations. Lane 1 - HeLa nuclear extract, Lane 2 - HeLa, Lane 3 - 293, Lane 4 - A431 and Lane 5 - Jurkat whole cell lysates. In lanes 6-10 the antibody was preincubated with 1 ug/ml of the immunizing peptide which effectively blocks the specific reactivity of this antibody with AP2A. Approximately 20 ug of each lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-AP2A antibody. Detection occurred using a 1:5000 dilution of HRP-labeled Rabbit anti-Goat IgG for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche) using a 60-sec exposure time.

Requested From: United States
Date Requested: 10/21/2016

Get Social With Us! Follow us on Facebook Follow us on Google+ Follow us on LinkedIn
Copyright © 2016 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy
Catalog Number