Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone 3H8] (IgG1) to Human TDP-43 / TARDBP
Human, Mouse, Rat, Bovine, Pig
IHC, IHC - Paraffin, Immunofluorescence, Western blot
Human TDP-43 / TARDBP
Human, Mouse, Rat, Bovine, Pig (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [3H8]
IHC (1:1000), IHC - Paraffin (1:50), Immunofluorescence (1:1000), Western blot (1:5000)
Specificity and Use
TDP-43 / TARDBP antibody was raised against full length recombinant human TDP43 expressed in and purified from E. coli.
Clone 3H8 is known to react with TDP43 from human, cow, pig, mouse, rat and other mammals. Since the TDP43 protein sequence is highly conserved across species barriers, it is likely that the antibody is effective on many other species also.
Try at dilutions of 1:1000 and higher for immunofluorescence, 1:5000 and lower for western blotting.
+4°C or -20°C, Avoid repeated freezing and thawing.
TDP-43 / TARDBP antibody was used to stain a section of formalin fixed adult rat brain, specifically the hippocampus. Hippocampal neuron nuclei are stained strongly. Chicken antibody to GFAP CPCA-GFAP (green) shows the processes of astrocytic glial cells. Nuclei of all cells are revealed with DAPI DNA stain (blue). The TARDP antibody stains neuronal nuclei strongly and the nuclei of some non-neuronal cells much more weakly. Neuronal nuclei therefore look crimson, since they are both red due to the content of TDP43 and blue due to their content of DNA, stained blue with DAPI.
Immunohistochemistry - Paraffin
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Blots of TDP-43 / TARDBP antibody on crude extract of mouse brain nuclear fraction (left lane) and cytoplasmic fraction (right lane) blotted with monoclonal antibody TDP-43 / TARDBP antibody. There is a strong clear band in the nuclear preparation running at 43kDa, and a much more minor band in the cytoplasmic fraction.
Requested From: United States
Date Requested: 5/22/2015