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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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STAR Antibody (clone 5F9) LS‑C198275
STAR antibody LS-C198275 is an unconjugated mouse monoclonal antibody to human STAR. Validated for ELISA, RNAi and WB.
Catalog
Size
Price
LS-C198275-100
100 µg
Unavailable

Popular STAR Products

STAR antibody detects STAR protein at cytosol on hepatoma by immunohistochemical analysis. Sample: Paraffin-embedded hepatoma. STAR antibody dilution:1:500.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
Western blot analysis of extracts of various cell lines.
Species: Human, Mouse
Applications: Immunofluorescence, Western blot
Immunohistochemistry of paraffin-embedded human testis tissue using antibody at dilution of 1:100.
Species: Human
Applications: IHC, IHC - Paraffin, ELISA
Immunohistochemistry of Human lung cancer using STAR Polyclonal Antibody at dilution of 1:35.
Species: Human, Mouse, Rat
Applications: IHC, Western blot, ELISA

Product Description

STAR antibody LS-C198275 is an unconjugated mouse monoclonal antibody to human STAR. Validated for ELISA, RNAi and WB.
About STAR
STAR plays a key role in the acute regulation of steroid hormone synthesis by enhancing the conversion of cholesterol into pregnenolone. This protein permits the cleavage of cholesterol into pregnenolone by mediating the transport of cholesterol from the outer mitochondrial membrane to the inner mitochondrial membrane. Mutations in this gene are a cause of congenital lipoid adrenal hyperplasia (CLAH), also called lipoid CAH. A pseudogene of this gene is located on chromosome 13. P49675 NM_000349 NP_000340.2

STAR Antibody, Cholesterol trafficker Antibody, START domain containing 1 Antibody, Steroidogenic acute regulator Antibody, STARD1 Antibody


Specifications

Target
Human STAR
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a,k Monoclonal [5F9]
Conjugations
Unconjugated
Purification
Purified from ascites by Protein A
Modifications
Unmodified
Applications
  • Western blot
  • ELISA
  • RNA interference
Immunogen
STAR antibody was raised against sTAR (AAH10550, aa81-180 partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human STAR
Usage
RNAi Knockdown: Antibody validated. Sandwich ELISA: Recombinant protein. Western Blot: Transfected lysate, Recombinant protein.
Presentation
PBS, pH 7.4
Storage
Store at -20°C or lower. Aliquot to avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

Western Blot analysis of STAR expression in transfected 293T cell line by STAR monoclonal antibody (M01), clone 5F9.Lane 1: STAR transfected lysate(31.9 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of STAR expression in transfected 293T cell line by STAR monoclonal antibody (M01), clone 5F9.Lane 1: STAR transfected lysate(31.9 KDa).Lane 2: Non-transfected lysate.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged STAR is approximately 0.03 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged STAR is approximately 0.03 ng/ml as a capture antibody.

RNA interference

Western blot analysis of STAR over-expressed 293 cell line, cotransfected with STAR Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with STAR monoclonal antibody (M01), clone 5F9 . GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western blot analysis of STAR over-expressed 293 cell line, cotransfected with STAR Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with STAR monoclonal antibody (M01), clone 5F9 . GAPDH ( 36.1 kDa ) used as specificity and loading control.

Western blot

Western Blot analysis of STAR expression in transfected 293T cell line by STAR monoclonal antibody (M01), clone 5F9.Lane 1: STAR transfected lysate(31.9 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of STAR expression in transfected 293T cell line by STAR monoclonal antibody (M01), clone 5F9.Lane 1: STAR transfected lysate(31.9 KDa).Lane 2: Non-transfected lysate.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged STAR is approximately 0.03 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged STAR is approximately 0.03 ng/ml as a capture antibody.

RNA interference

Western blot analysis of STAR over-expressed 293 cell line, cotransfected with STAR Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with STAR monoclonal antibody (M01), clone 5F9 . GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western blot analysis of STAR over-expressed 293 cell line, cotransfected with STAR Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with STAR monoclonal antibody (M01), clone 5F9 . GAPDH ( 36.1 kDa ) used as specificity and loading control.

Western blot

Western Blot analysis of STAR expression in transfected 293T cell line by STAR monoclonal antibody (M01), clone 5F9.Lane 1: STAR transfected lysate(31.9 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of STAR expression in transfected 293T cell line by STAR monoclonal antibody (M01), clone 5F9.Lane 1: STAR transfected lysate(31.9 KDa).Lane 2: Non-transfected lysate.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged STAR is approximately 0.03 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged STAR is approximately 0.03 ng/ml as a capture antibody.

RNA interference

Western blot analysis of STAR over-expressed 293 cell line, cotransfected with STAR Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with STAR monoclonal antibody (M01), clone 5F9 . GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western blot analysis of STAR over-expressed 293 cell line, cotransfected with STAR Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with STAR monoclonal antibody (M01), clone 5F9 . GAPDH ( 36.1 kDa ) used as specificity and loading control.

Western blot

Western Blot analysis of STAR expression in transfected 293T cell line by STAR monoclonal antibody (M01), clone 5F9.Lane 1: STAR transfected lysate(31.9 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of STAR expression in transfected 293T cell line by STAR monoclonal antibody (M01), clone 5F9.Lane 1: STAR transfected lysate(31.9 KDa).Lane 2: Non-transfected lysate.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged STAR is approximately 0.03 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged STAR is approximately 0.03 ng/ml as a capture antibody.

RNA interference

Western blot analysis of STAR over-expressed 293 cell line, cotransfected with STAR Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with STAR monoclonal antibody (M01), clone 5F9 . GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western blot analysis of STAR over-expressed 293 cell line, cotransfected with STAR Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with STAR monoclonal antibody (M01), clone 5F9 . GAPDH ( 36.1 kDa ) used as specificity and loading control.


Requested From: 
Date Requested: 6/19/2018

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