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Anti-SQSTM1 Antibody (clone 4C8) LS-C172484

Ordering

Wt. Vol. Conc. Price
- 100 µl 0.95 mg/ml $325
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular SQSTM1 Antibodies

Anti-SQSTM1 Antibody (aa421-440) LS-C37984
Guinea pig Polyclonal (IgG) to Human SQSTM1
Human, Mouse, Rat, Bat, Bovine, Hamster, Horse, Pig, Rabbit, Sheep
IHC - Paraffin, IHC - Frozen, ICC, Western blot
Unconjugated
Anti-SQSTM1 Antibody (C-Terminus) IHC-plus™ LS-B4617
Rabbit Polyclonal to Human SQSTM1
Human, Mouse, Rat
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-SQSTM1 Antibody (aa323-372) IHC-plus™ LS-B5839
Rabbit Polyclonal to Human SQSTM1
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Sheep, Chicken
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 4C8] (IgG2a) to Human SQSTM1
Human
Immunofluorescence, Western blot, Flow Cytometry
Unconjugated

Details

Human SQSTM1
Mouse
Human (tested or 100% immunogen sequence identity)
IgG2a Monoclonal [4C8]
Unconjugated
Protein A/G purified
Unmodified

Applications

  • Immunofluorescence (1:100)
  • Western blot (1:1000 - 1:2000)
  • Flow Cytometry (1:100)

Specificity and Use

SQSTM1 antibody was raised against full length human recombinant protein of human SQSTM1 (NP_003891) produced in HEK293T cell.
Human P62 / SQSTM1

Packaging

PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Store at -20°C. Minimize freezing and thawing.
For research use only.

About SQSTM1

Q13501 NM_003900 NP_003891.1

SQSTM1 Antibody, A170 Antibody, ORCA Antibody, OSIL Antibody, Paget disease of bone 3 Antibody, p60 Antibody, p62 Antibody, PDB3 Antibody, Sequestosome-1 Antibody, Ubiquitin-binding protein p62 Antibody, Oxidative stress induced like Antibody, p62B Antibody, ZIP3 Antibody, Sequestosome 1 Antibody, EBI3-associated protein p60 Antibody, EBIAP Antibody

Autophagy receptor that interacts directly with both the cargo to become degraded and an autophagy modifier of the MAP1 LC3 family. Required both for the formation and autophagic degradation of polyubiquitin-containing bodies, called ALIS (aggresome-like induced structures) and links ALIS to the autophagic machinery. Involved in midbody ring degradation. May regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1.

Immunofluorescence

Immunofluorescence
Anti-SQSTM1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SQSTM1.

Western blot

Western blot
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SQSTM1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SQSTM1.

Western blot

Western blot
Western blot of extracts (35 ug) from 9 different cell lines by using anti-SQSTM1 monoclonal antibody.

Flow Cytometry

Flow Cytometry
Flow cytometry of HeLa cells, using anti-SQSTM1 antibody (Red), compared to a nonspecific negative control antibody (Blue).

Flow Cytometry

Flow Cytometry
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SQSTM1 antibody, and then analyzed by flow cytometry.
 

Requested From: United States
Date Requested: 12/8/2016

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