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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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SQSTM1 Antibody (aa323‑372) IHC‑plus™ LS‑B5839
Note: This antibody replaces LS-C111548
SQSTM1 antibody LS-B5839 is an unconjugated rabbit polyclonal antibody to SQSTM1 from human, mouse, rat and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B5839-50
50 µg (1 mg/ml)
Unavailable

Popular SQSTM1 Products

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Applications: IHC, IHC - Paraffin, IHC - Frozen, ICC, Western blot
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Product Description

SQSTM1 antibody LS-B5839 is an unconjugated rabbit polyclonal antibody to SQSTM1 from human, mouse, rat and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
About SQSTM1
Autophagy receptor that interacts directly with both the cargo to become degraded and an autophagy modifier of the MAP1 LC3 family. Required both for the formation and autophagic degradation of polyubiquitin-containing bodies, called ALIS (aggresome-like induced structures) and links ALIS to the autophagic machinery. Involved in midbody ring degradation. May regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. Q13501 NM_003900 NP_003891.1

SQSTM1 Antibody, A170 Antibody, ORCA Antibody, OSIL Antibody, Paget disease of bone 3 Antibody, p60 Antibody, p62 Antibody, PDB3 Antibody, Sequestosome-1 Antibody, Ubiquitin-binding protein p62 Antibody, Oxidative stress induced like Antibody, p62B Antibody, ZIP3 Antibody, Sequestosome 1 Antibody, EBI3-associated protein p60 Antibody, EBIAP Antibody


Specifications

Target
Human SQSTM1
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Sheep, Chicken (tested or 100% immunogen sequence identity)
Predicted
Xenopus, Zebrafish (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
SQSTM1 antibody was raised against synthetic peptide located between aa323-372 of human SQSTM1 (Q13501, NP_003891). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Sheep, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus (100%); Xenopus, Salmon, Stickleback, Zebrafish (92%).
Blocking Peptide
LS-E19515 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B5839. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa323-372
Specificity
Human SQSTM1
Usage
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Anti-SQSTM1 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5839 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-SQSTM1 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5839 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

SQSTM1 antibody LS-B5839 Western blot of Fetal Pancreas lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
SQSTM1 antibody LS-B5839 Western blot of Fetal Pancreas lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-SQSTM1 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5839 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-SQSTM1 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5839 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

SQSTM1 antibody LS-B5839 Western blot of Fetal Pancreas lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
SQSTM1 antibody LS-B5839 Western blot of Fetal Pancreas lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-SQSTM1 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5839 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-SQSTM1 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5839 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

SQSTM1 antibody LS-B5839 Western blot of Fetal Pancreas lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
SQSTM1 antibody LS-B5839 Western blot of Fetal Pancreas lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-SQSTM1 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5839 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-SQSTM1 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5839 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

SQSTM1 antibody LS-B5839 Western blot of Fetal Pancreas lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
SQSTM1 antibody LS-B5839 Western blot of Fetal Pancreas lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/24/2018

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