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Anti-SQSTM1 Antibody (aa317-346) LS-C161484

Ordering

Wt. Vol. Conc. Price
- 400 µl - $305
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular SQSTM1 Antibodies

Anti-SQSTM1 Antibody (aa421-440) LS-C37984
Guinea pig Polyclonal (IgG) to Human SQSTM1
Human, Mouse, Rat, Bat, Bovine, Hamster, Horse, Pig, Rabbit, Sheep
IHC - Paraffin, IHC - Frozen, ICC, Western blot
Unconjugated
Anti-SQSTM1 Antibody (C-Terminus) IHC-plus™ LS-B4617
Rabbit Polyclonal to Human SQSTM1
Human, Mouse, Rat
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-SQSTM1 Antibody (aa323-372) IHC-plus™ LS-B5839
Rabbit Polyclonal to Human SQSTM1
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Sheep, Chicken
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed Publications Publications (2)
Rabbit Polyclonal to Human SQSTM1
Human
IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated

Details

Human SQSTM1
Rabbit
Human (tested or 100% immunogen sequence identity)
Mouse, Rat (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated
Protein A purified
Unmodified

Applications

  • IHC - Paraffin (1:50 - 1:100)
  • Immunofluorescence (1:50 - 1:100)
  • Western blot (1:1000)

Specificity and Use

LS-E6926 - Lyophilized - 100 µg - $145.00
aa317-346
This SQSTM1 (p62) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 317-346 amino acids from the C-terminal region of human SQSTM1 (p62).

Packaging

PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

Publications (2)

1
Impaired protein aggregate handling and clearance underlie the pathogenesis of p97/VCP-associated disease. Ju JS, Miller SE, Hanson PI, Weihl CC. The Journal of biological chemistry. 2008 283:30289-99. [PubMed:18715868] [PMC:PMC2573070]
2
Overexpression of the autophagic beclin-1 protein clears mutant ataxin-3 and alleviates Machado-Joseph disease. Nascimento-Ferreira I, Santos-Ferreira T, Sousa-Ferreira L, Auregan G, Onofre I, Alves S, Dufour N, Colomer Gould VF, Koeppen A, Dglon N, Pereira de Almeida L. Brain : a journal of neurology. 2011 134:1400-15. [PubMed:21478185]

About SQSTM1

Q13501 NM_003900 NP_003891.1

SQSTM1 Antibody, A170 Antibody, ORCA Antibody, OSIL Antibody, Paget disease of bone 3 Antibody, p60 Antibody, p62 Antibody, PDB3 Antibody, Sequestosome-1 Antibody, Ubiquitin-binding protein p62 Antibody, Oxidative stress induced like Antibody, p62B Antibody, ZIP3 Antibody, Sequestosome 1 Antibody, EBI3-associated protein p60 Antibody, EBIAP Antibody

Autophagy receptor that interacts directly with both the cargo to become degraded and an autophagy modifier of the MAP1 LC3 family. Required both for the formation and autophagic degradation of polyubiquitin-containing bodies, called ALIS (aggresome-like induced structures) and links ALIS to the autophagic machinery. Involved in midbody ring degradation. May regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1.

Immunohistochemistry

Immunohistochemistry
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Immunofluorescence

Immunofluorescence
Immunofluorescence staining of Autophagy SQSTM1 (p62) Antibody on Methanol-fixed and PFA fixed HeLa cells. Data courtesy of Dr. Eeva-Liisa Eskelinen, University of Helsinki,Finland.

Immunofluorescence

Immunofluorescence
Fluorescent image of U251 cells stained with SQSTM1 (p62) antibody. U251 cells were treated with Chloroquine (50 mu M,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated SQSTM1 (p62) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). SQSTM1 (p62) immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000161011).

Western blot

Western blot
Western blot of SQSTM1 (arrow) using rabbit polyclonal Autophagy SQSTM1 (p62) Antibody (C-term ). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SQSTM1 gene (Lane 2) (Origene Technologies).

Western blot

Western blot
Western blot of SQSTM1 Antibody (C331) antibody pre-incubated without(lane 1) and with(lane 2) blocking peptide in MCF-7 cell line lysate. SQSTM1 Antibody (C331) (arrow) was detected using the purified antibody.
 

Requested From: United States
Date Requested: 1/20/2017

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