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Anti-SMURF2 Antibody (aa702-731) LS-C98006

Ordering

Wt. Vol. Conc. Price
- 400 µl - Unavailable

Most Popular SMURF2 Antibodies

Anti-SMURF2 Antibody (aa161-174) LS-C10240
Rabbit Polyclonal (IgG) to Human SMURF2
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Rabbit, Chicken, Xenopus, Zebrafish
Western blot
Unconjugated
Anti-SMURF2 Antibody (aa6-36) LS-C98005
Rabbit Polyclonal to Human SMURF2
Human, Mouse
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-SMURF2 Antibody (aa702-731) LS-C98006
Rabbit Polyclonal to Human SMURF2
Human
IHC - Paraffin, ICC, Immunofluorescence, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human SMURF2
Human
IHC - Paraffin, ICC, Immunofluorescence, Western blot
Unconjugated

Details

Human SMURF2
Rabbit
Human (tested or 100% immunogen sequence identity)
Mouse, Xenopus (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated
Ammonium sulfate precipitation
Unmodified

Applications

  • IHC - Paraffin (1:50 - 1:100)
  • ICC
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)

Specificity and Use

LS-E6855 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C98006. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
aa702-731
This SMURF2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 702-731 amino acids from the C-terminal region of human SMURF2.

Packaging

PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

About SMURF2

Q9HAU4 NM_022739 NP_073576.1

SMURF2 Antibody, E3 ubiquitin ligase SMURF2 Antibody, HSMURF2 Antibody

E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Interacts with SMAD1 and SMAD7 in order to trigger their ubiquitination and proteasome-dependent degradation. In addition, interaction with SMAD7 activates autocatalytic degradation, which is prevented by interaction with SCYE1.

Immunohistochemistry

Immunohistochemistry
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Immunofluorescence

Immunofluorescence
Hippocampal neurons were fixed at stage 3, stained with anti-Smurf2 (red) and anti-Kinesin-2 (green) antibodies, and analyzed by confocal microscopy. The panels show single confocal planes. (J. Biol. Chem. 2007 Nov 30;282(48):35259-35268)

Immunofluorescence

Immunofluorescence
Hippocampal neurons were transfected 2 h after plating with expression vectors for EGFP, EGFP-tagged Par3-4N/2, Par3-PDZ2, Par3-PDZ3, Smurf2-HECT (HECT), Smurf2-HECT-C716A (HECT CA), and shRNA directed against mPar3 (Par3 RNAi), or vectors for the anti-Par3 shRNA and human Myc-Par3 (RNAi + h Par3) (green). Transfected cells were analyzed at 3 d.i.v. by staining with an anti-Smurf2 antibody (red). Axons are marked by arrowheads. The marked growth cones are shown at a higher magnification. Scale bars, 40 and 10 ?. (J. Biol. Chem. 2007 Nov 30;282(48):35259-35268)

Western blot

Western blot
Western blot of anti-SMURF2 antibody in 293 cell line lysate (35 ug/lane). SMURF2(arrow) was detected using the purified Pab

Requested From: 
Date Requested: 3/28/2017

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