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Anti-SMC4 Antibody (aa493-504) LS-C12387

Ordering

Wt. Vol. Conc. Price
200 µg - - $485
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
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Rabbit Polyclonal (IgG) to Human SMC4
Human, Monkey, Bat, Bovine, Dog, Horse, Pig, Rabbit, Xenopus
Western blot
Unconjugated

Details

Human SMC4
Rabbit
Human, Monkey, Bat, Bovine, Dog, Horse, Pig, Rabbit, Xenopus (tested or 100% immunogen sequence identity)
Mouse, Rat (at least 90% immunogen sequence identity)
IgG Polyclonal
Protein A purified

Applications

Western blot

Specificity and Use

SMC4 antibody was raised against kLH-conjugated, synthetic peptide corresponding to amino acids 493-504 (C-NEARSKMDVAQS) of human CAP-C. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Elephant, Panda, Bovine, Dog, Bat, Horse, Rabbit, Pig, Opossum, Platypus, Xenopus (100%); Mouse, Rat (92%); Turkey, Chicken, Zebrafish (83%).
aa493-504
Recognizes CAP-C, Mr 165kD. Species cross-reactivity: Human, rat and mouse.
Western Blot: 0.5-2 ug/ul. HeLa Nuclear Extract probed with anti-CAP-C (0.5 ug/ul). Positive Antigen Control: HeLa nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 5-10 ul of extract and boil for 5 minutes to reduce the preparation. Load 20 ug of reduced lysate per lane for minigels.

Packaging

0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05% sodium azide, 30% glycerol.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About SMC4

Q9NTJ3 NM_005496 NP_005487.3

SMC4 Antibody, HCAP-C Antibody, SMC protein 4 Antibody, SMC4L1 Antibody, SMC-4 Antibody, XCAP-C homolog Antibody, CAP-C Antibody, CAPC Antibody

Central component of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases.

Requested From: United States
Date Requested: 9/30/2016

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