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Catalog Number Size Price
LS-C287004-10 10 µl $295 
LS-C287004-100 100 µl (1 mg/ml) $514 
SMC4 Antibody - Localization of SMC4 to Mitotic Chromosomes by Immunofluorescence. Samples: HeLa cells grown on coverslips, fixed with methanol/acetone and blocked with 3% BSA in PBS. Antibody: Affinity purified rabbit anti-SMC4 antibody used at 5 ug/ml. Detection: Rhodamine labeled anti-rabbit secondary antibody. Cells were counterstained with DAPI.
SMC4 Antibody - Detection of human SMC4 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SMC4 antibody used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
SMC4 Antibody - Detection of Human SMC4 by Western Blot. Samples: Whole cell lysate (90 ug) from 293T cells (lane 1), SMC4 transfected 239T cells (lane 2) or normal human lymphoblasts (lane 3) separated on an 8% SDS-PAGE gel. Antibodies: Affinity purified rabbit anti-SMC4 used 0.2 ug/ml and goat anti-rabbit HRP used at a dilution of 1:20000. Detection: Chemiluminescence with 5 second.
SMC4 Antibody - Localization of SMC4 to Mitotic Chromosomes by Immunofluorescence. Samples: HeLa cells grown on coverslips, fixed with methanol/acetone and blocked with 3% BSA in PBS. Antibody: Affinity purified rabbit anti-SMC4 antibody used at 5 ug/ml. Detection: Rhodamine labeled anti-rabbit secondary antibody. Cells were counterstained with DAPI.
SMC4 Antibody - Detection of human SMC4 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SMC4 antibody used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
SMC4 Antibody - Detection of Human SMC4 by Western Blot. Samples: Whole cell lysate (90 ug) from 293T cells (lane 1), SMC4 transfected 239T cells (lane 2) or normal human lymphoblasts (lane 3) separated on an 8% SDS-PAGE gel. Antibodies: Affinity purified rabbit anti-SMC4 used 0.2 ug/ml and goat anti-rabbit HRP used at a dilution of 1:20000. Detection: Chemiluminescence with 5 second.
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Polyclonal Rabbit anti‑Human SMC4 Antibody (aa600‑650, IF, WB) LS‑C287004

Polyclonal Rabbit anti‑Human SMC4 Antibody (aa600‑650, IF, WB) LS‑C287004

Antibody:
SMC4 Rabbit anti-Human Polyclonal (aa600-650) Antibody
Application:
ICC, IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$295
LS-C287004-10
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
SMC4 Rabbit anti-Human Polyclonal (aa600-650) Antibody
Application:
ICC, IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
SMC4 antibody LS-C287004 is an unconjugated rabbit polyclonal antibody to human SMC4 (aa600-650). Validated for ICC, IF and WB.
Target
Human SMC4
Synonyms
SMC4 | HCAP-C | SMC protein 4 | SMC4L1 | SMC-4 | XCAP-C homolog | CAP-C | CAPC
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Mouse (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
A portion of GeneID: 10051 corresponding to amino acids between 600 and 650 of UniprotID: Q9NTJ3
Epitope
aa600-650
Specificity
Region between residues 600 and 650 of human Structural Maintenance of Chromosomes 4 using the numbering given in entry NP_005487.2 (GeneID 10051).
Applications
  • ICC (1:500 - 1:2000)
  • Immunofluorescence (1:500 - 1:2000)
  • Western blot (1:1000 - 1:10000)
Usage
IHC-IF: 1:500-1:2000
Manufacturer
Bethyl Laboratories, Inc.
Presentation
Tris-citrate/phosphate buffer, pH 7-8, 0.09% sodium azide.
Storage
Store at 2-8°C for up to one year.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SMC4
Q9NTJ3 NM_005496 NP_005487.3

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 9/19/2024