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Anti-SMAD5 Antibody (clone 3H9) LS-C169300

Ordering

Wt. Vol. Conc. Price
- 100 µl - $345
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular SMAD5 Antibodies

Anti-SMAD5 Antibody (aa241-269, AP) LS-C269625
Rabbit Polyclonal (IgG) to Human SMAD5
Human
Western blot, ELISA
AP Conjugated

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 3H9] (IgG1) to Human SMAD5
Human, Rat
IHC - Paraffin, ICC, Immunofluorescence, Western blot, Flow Cytometry, ELISA
Unconjugated

Details

Human SMAD5
Mouse
Human, Rat (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [3H9]
Unconjugated
Ascites
Unmodified

Applications

  • IHC - Paraffin (1:200 - 1:1000)
  • ICC (1:200 - 1:1000)
  • Immunofluorescence (1:1000)
  • Western blot (1:500 - 1:2000)
  • Flow Cytometry (1:200 - 1:400)
  • ELISA (1:10000)

Specificity and Use

SMAD5 antibody was raised against purified recombinant fragment of human SMAD5 expressed in E. coli.
Human SMAD5

Packaging

Ascites, 0.03% sodium azide
Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.
For research use only.

About SMAD5

Q99717 NM_005903 NP_005894.3

SMAD5 Antibody, JV5-1 Antibody, MADH5 Antibody, SMAD 5 Antibody, MAD homolog 5 Antibody, Mothers against DPP homolog 5 Antibody, SMA- and MAD-related protein 5 Antibody, SMAD family member 5 Antibody, DWFC Antibody, HSmad5 Antibody

SMAD5 is involved in the transforming growth factor beta signaling pathway that results in an inhibition of the proliferation of hematopoietic progenitor cells. The encoded protein is activated by bone morphogenetic proteins type 1 receptor kinase, and may be involved in cancer. Alternative splicing results in multiple transcript variants.

Immunohistochemistry

Immunohistochemistry
IHC of paraffin-embedded brain tissues (left) and lung cancer tissues (right) using SMAD5 mouse monoclonal antibody with DAB staining.

Immunofluorescence

Immunofluorescence
Immunofluorescence of NTERA-2 cells using SMAD5 mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Western blot

Western blot
Western blot using SMAD5 mouse monoclonal antibody against HeLa (1), SK-N-SH (2), PC-12 (3), Jurkat (4), and K562 (5) cell lysate.
 

Requested From: United States
Date Requested: 12/5/2016

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