Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Rabbit Polyclonal (IgG) to Rat SLC4A1 / Band 3 / AE1
Western blot, ELISA
Rat SLC4A1 / Band 3 / AE1
Rat (tested or 100% immunogen sequence identity)
Mouse (at least 90% immunogen sequence identity)
Western blot (1:1000 - 1:5000)
ELISA (1:10000 - 1:50000)
Specificity and Use
An 20 AA Peptide sequence near the N-terminus of rat AE-1 (1). The peptide was coupled to KLH.
Recognizes rat Anion Exchanger 1 (AE1). Species sequence homology: mouse 90%; human 75%; bovine 70%. No significant sequence homology is detected with other AE or other proteins.
Suitable for use in ELISA and Western Blot. Western Blot: 1:1000-1:5000. AE1 is approx 90-100kD proteins. It aggregates easily forming dimers and multimers. It may also be proteolytically processed to smaller fragments of 60, 40, 20kD. ELISA: 1:10000-1:50000. Control peptide can be used to coat ELISA plates at 1 ug/ml.
PBS, 0.05% sodium azide.
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
SLC4A1 Antibody, Anion exchanger 1 Antibody, Anion exchanger-1 Antibody, AE1 Antibody, Anion exchange protein 1 Antibody, Band 3 Antibody, Band 3 anion transport protein Antibody, BND3 Antibody, CD233 Antibody, DI Antibody, EPB3 Antibody, AE 1 Antibody, Froese blood group Antibody, FR Antibody, RTA1A Antibody, SW Antibody, Swann blood group Antibody, WD1 Antibody, WR Antibody, Wright blood group Antibody, WD Antibody, CD233 antigen Antibody, EMPB3 Antibody, Waldner blood group Antibody
SLC4A1 / Band 3 / AE1 is part of the anion exchanger (AE) family and is expressed in the erythrocyte plasma membrane, where it functions as a chloride/bicarbonate exchanger involved in carbon dioxide transport from tissues to lungs. The protein comprises two domains that are structurally and functionally distinct. The N-terminal 40kDa domain is located in the cytoplasm and acts as an attachment site for the red cell skeleton by binding ankyrin.