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Anti-SLC25A28 Antibody (aa290-339) IHC-plus™ LS-B3556

Note: This antibody replaces LS-C80633

Ordering

Wt. Vol. Conc. Price
50 µg - - $450
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular SLC25A28 Antibodies

Anti-SLC25A28 Antibody (aa290-339) IHC-plus™ LS-B3556
Rabbit Polyclonal (IgG) to Human SLC25A28
Human, Monkey, Rat, Bovine
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-SLC25A28 Antibody (aa44-74, APC) LS-C207233
Rabbit Polyclonal (IgG) to Human SLC25A28
Human
IHC, Western blot, Flow Cytometry, ELISA
APC Conjugated
Anti-SLC25A28 Antibody (aa44-74, Azide-free, HRP) LS-C207236
Rabbit Polyclonal (IgG) to Human SLC25A28
Human
IHC, Western blot, Flow Cytometry, ELISA
HRP Conjugated, Azide-free

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human SLC25A28
Human, Monkey, Rat, Bovine
IHC - Paraffin, Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human SLC25A28
Rabbit
Human, Monkey, Rat, Bovine (tested or 100% immunogen sequence identity)
Mouse, Dog, Guinea pig, Horse, Pig, Rabbit, Chicken (at least 90% immunogen sequence identity)
IgG Polyclonal
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot
  • (applications tested for the base form of this product only)

Specificity and Use

SLC25A28 antibody was raised against synthetic peptide located between aa290-339 of human SLC25A28 (Q96A46, NP_112489). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset (100%); Galago, Mouse, Elephant, Panda, Dog, Rabbit, Horse, Pig, Guinea pig, Chicken (92%); Rat, Bovine, Bat, Opossum, Platypus (85%).
LS-E16193 - Lyophilized - 100 µg - $145.00
aa290-339
Human SLC25A28
Immunohistochemistry: LS-B3556 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3556 was determined to be 5 ug/ml. ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Packaging

Lyophilized from PBS with 2% sucrose
Distilled Water.
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About SLC25A28

Q96A46 NM_031212 NP_112489.3

SLC25A28 Antibody, Mitoferrin 2 Antibody, MRS4L Antibody, HMRS3/4 Antibody, MFRN2 Antibody, MRS3/4 Antibody, Mitoferrin-2 Antibody, NPD016 Antibody

Mitochondrial iron transporter that mediates iron uptake. Probably required for heme synthesis of hemoproteins and Fe-S cluster assembly in non-erythroid cells. The iron delivered into the mitochondria, presumably as Fe2+, is then probably delivered to ferrochelatase to catalyze Fe2+ incorporation into protoprophyrin IX to make heme.

Immunohistochemistry

Immunohistochemistry
Anti-SLC25A28 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3556 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Immunohistochemistry
Anti-SLC25A28 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3556 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
MCF7 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.
 

Requested From: United States
Date Requested: 12/4/2016

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