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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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SIX1 Antibody (clone 1H4) LS‑C173609
SIX1 antibody LS-C173609 is an unconjugated mouse monoclonal antibody to human SIX1. Validated for WB.
Catalog
Size
Price
LS-C173609-100
100 µl (0.77 mg/ml)
Unavailable

Popular SIX1 Products

Antibody
Species: Mouse
Applications: IHC, ICC, Western blot, Immunoprecipitation, ELISA, Competition
Antibody
Species: Human, Mouse
Applications: Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Antibody
Species: Human, Mouse
Applications: Western blot, ELISA
All lanes: Anti-SIX1 Antibody (Center) at 1:2000 dilution. Lane 1: Jurkat whole cell lysate. Lane 2: NIH/3T3 whole cell lysate. Lane 3: U-2OS whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 32 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Species: Human, Mouse
Applications: Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Immunohistochemistry of paraffin-embedded human skeletal muscle tissue using CSB-PA618986LA01HU at dilution of 1:100
Species: Human, Rat
Applications: IHC, Western blot, ELISA

Product Description

SIX1 antibody LS-C173609 is an unconjugated mouse monoclonal antibody to human SIX1. Validated for WB.
About SIX1
Transcription factor that is involved in the regulation of cell proliferation, apoptosis and embryonic development. Plays an important role in the development of several organs, including kidney, muscle and inner ear. Depending on context, functions as transcriptional repressor or activator. Lacks an activation domain, and requires interaction with EYA family members for transcription activation. Mediates nuclear translocation of EYA1 and EYA2. Q15475 NM_005982 NP_005973.1

SIX1 Antibody, BOS3 Antibody, DFNA23 Antibody, Sine oculis homeobox homolog 1 Antibody, SIX homeobox 1 Antibody, TIP39 Antibody, Homeobox protein SIX1 Antibody


Specifications

Target
Human SIX1
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal [1H4]
Conjugations
Unconjugated
Purification
Protein A/G purified
Modifications
Unmodified
Applications
  • Western blot (1:2000)
Immunogen
SIX1 antibody was raised against human recombinant protein fragment corresponding to amino acids 1-284 of human SIX1(NP_005973) produced in E. coli.
Specificity
Human SIX1
Presentation
PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SIX1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SIX1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SIX1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SIX1.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SIX1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SIX1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SIX1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SIX1.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SIX1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SIX1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SIX1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SIX1.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SIX1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SIX1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SIX1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SIX1.


Requested From: 
Date Requested: 6/24/2018

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