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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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SIX1

SIX homeobox 1

Transcription factor that is involved in the regulation of cell proliferation, apoptosis and embryonic development. Plays an important role in the development of several organs, including kidney, muscle and inner ear. Depending on context, functions as transcriptional repressor or activator. Lacks an activation domain, and requires interaction with EYA family members for transcription activation. Mediates nuclear translocation of EYA1 and EYA2. Binds the 5'-TCA[AG][AG]TTNC-3' motif present in the MEF3 element in the MYOG promoter. Regulates the expression of numerous genes, including MYC, CCND1 and EZR. Acts as activator of the IGFBP5 promoter, probably coactivated by EYA2. Repression of precursor cell proliferation in myoblasts is switched to activation through recruitment of EYA3 to the SIX1-DACH1 complex. During myogenesis, seems to act together with EYA2 and DACH2 (By similarity). Regulates the expression of CCNA1.

Gene Name: SIX homeobox 1
Synonyms: SIX1, BOS3, DFNA23, Sine oculis homeobox homolog 1, SIX homeobox 1, TIP39, Homeobox protein SIX1
Target Sequences: NM_005982 NP_005973.1 Q15475

Filtered By: None
Target
SIX1 (22)
Reactivity
Human (21)
Mouse (9)
Rat (6)
Bat (4)
Bovine (4)
Chimpanzee (6)
Dog (4)
Gibbon (3)
Guinea pig (4)
Hamster (4)
Horse (4)
Monkey (4)
Pig (8)
Sheep (4)
Application
IHC (2)
WB (18)
Compet (1)
ELISA (7)
ICC (1)
IP (1)
Peptide-ELISA (2)
Host
rabbit (20)
mouse (2)
Isotype
IgG (13)
IgG1 (1)
IgG2a,k (1)
Clonality
monoclonal mc (2)
polyclonal pc (20)
Clone
1H4 (1)
3C7 (1)
Format
Biotin Conjugated (3)
FITC Conjugated (3)
HRP Conjugated (3)
Unconjugated (13)
Epitope
C-Terminus (8)
aa111-160 (1)
aa245-282 (1)
aa69-102 (1)
aa80-160 (1)
Publications
No (22)
Showing 1-22 of 22 results

SIX1 Antibody LS-C139037
Rabbit Polyclonal to Mouse SIX1
Compet ELISA ICC IHC IP WB
Unconjugated
50µg  $365.00

SIX1 Antibody LS-C500844
Rabbit Polyclonal (IgG) to Human SIX1
Rat, Human
ELISA IHC WB
Unconjugated
50µg  $285.00; 100µg  $385.00
Immunohistochemistry of paraffin-embedded human skeletal muscle tissue using CSB-PA618986LA01HU at dilution of 1:100
Positive WB detected in:LO2 whole cell lysate,PC3 whole cell lysate,NIH/3T3 whole cell lysate,Rat liver tissue;All lanes:SIX1 antibody at 2.7?g/ml;Secondary;Goat polyclonal to rabbit IgG at 1/50000 dilution;Predicted band size: 33 KDa;Observed band size: 33 KDa;
Immunohistochemistry of paraffin-embedded human skeletal muscle tissue using CSB-PA618986LA01HU at dilution of 1:100
Positive WB detected in:LO2 whole cell lysate,PC3 whole cell lysate,NIH/3T3 whole cell lysate,Rat liver tissue;All lanes:SIX1 antibody at 2.7?g/ml;Secondary;Goat polyclonal to rabbit IgG at 1/50000 dilution;Predicted band size: 33 KDa;Observed band size: 33 KDa;

SIX1 Antibody (HRP) LS-C500849
Rabbit Polyclonal (IgG) to Human SIX1
Human
ELISA
HRP Conjugated
50µg  $285.00; 100µg  $385.00

SIX1 Antibody (Biotin) LS-C500847
Rabbit Polyclonal (IgG) to Human SIX1
Human
ELISA
Biotin Conjugated
50µg  $285.00; 100µg  $385.00

SIX1 Antibody (FITC) LS-C500851
Rabbit Polyclonal (IgG) to Human SIX1
Human
ELISA
FITC Conjugated
50µg  $285.00; 100µg  $385.00

SIX1 Antibody (aa80-160) LS-C386067
Rabbit Polyclonal (IgG) to Human SIX1
Mouse, Human
ELISA WB
Unconjugated
100µl 1mg/ml  $265.00

SIX1 Antibody (aa111-160) LS-C291338
Rabbit Polyclonal (IgG) to Human SIX1
Mouse, Human
Peptide-ELISA WB
Unconjugated
50µl 1mg/ml  $265.00; 100µl 1mg/ml  $325.00

SIX1 Antibody (aa69-102) LS-C392817
Rabbit Polyclonal to Human SIX1
Mouse, Human
Peptide-ELISA WB
Unconjugated
50µl  $205.00; 200µl  $305.00
All lanes: Anti-SIX1 Antibody (Center) at 1:2000 dilution. Lane 1: Jurkat whole cell lysate. Lane 2: NIH/3T3 whole cell lysate. Lane 3: U-2OS whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 32 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Anti-SIX1 Antibody (Center) at 1:2000 dilution. Lane 1: Jurkat whole cell lysate. Lane 2: NIH/3T3 whole cell lysate. Lane 3: U-2OS whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 32 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

SIX1 Antibody (clone 3C7) LS-C198200
Mouse Monoclonal [clone 3C7] (IgG2a,k) to Human SIX1
Human
ELISA
Unconjugated
100µg  $355.00
Detection limit for recombinant GST tagged SIX1 is approximately 3 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged SIX1 is approximately 3 ng/ml as a capture antibody.

SIX1 Antibody (clone 1H4) LS-C173609
Mouse Monoclonal [clone 1H4] (IgG1) to Human SIX1
Human
WB
Unconjugated
100µl 0.77mg/ml  $345.00
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SIX1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SIX1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SIX1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SIX1.

SIX1 Antibody (C-Terminus) LS-C101389
Rabbit Polyclonal (IgG) to Human SIX1
Mouse, Dog, Guinea pig, Sheep, Bovine, Rat, Hamster, Pig, Horse, Bat, Human, Monkey
WB
Unconjugated
50µg  $365.00
SIX1 antibody LS-C101389 Western blot of 721_B cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
SIX1 antibody LS-C101389 Western blot of 721_B cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

SIX1 Antibody (C-Terminus, HRP) LS-C427779
Rabbit Polyclonal (IgG) to Human SIX1
Mouse, Dog, Guinea pig, Sheep, Bovine, Pig, Horse, Bat, Rat, Hamster, Chimpanzee, Gibbon, Human, Monkey
WB
HRP Conjugated
100µl 0.65mg/ml  $385.00
SIX1 antibody LS-C101389 Western blot of 721_B cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
SIX1 antibody LS-C101389 Western blot of 721_B cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

SIX1 Antibody (C-Terminus, Biotin) LS-C427777
Rabbit Polyclonal (IgG) to Human SIX1
Mouse, Dog, Guinea pig, Sheep, Bovine, Pig, Horse, Bat, Rat, Hamster, Chimpanzee, Gibbon, Human, Monkey
WB
Biotin Conjugated
100µl 1mg/ml  $385.00
SIX1 antibody LS-C101389 Western blot of 721_B cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
SIX1 antibody LS-C101389 Western blot of 721_B cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

SIX1 Antibody (C-Terminus, FITC) LS-C427778
Rabbit Polyclonal (IgG) to Human SIX1
Mouse, Dog, Guinea pig, Sheep, Bovine, Pig, Horse, Bat, Rat, Hamster, Chimpanzee, Gibbon, Human, Monkey
WB
FITC Conjugated
100µl 1mg/ml  $385.00
SIX1 antibody LS-C101389 Western blot of 721_B cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
SIX1 antibody LS-C101389 Western blot of 721_B cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

SIX1 Antibody (C-Terminus) LS-C110311
Rabbit Polyclonal to Human SIX1
Pig, Human
WB
Unconjugated
50µg  $365.00
SIX1 antibody LS-C110311 Western blot of PANC1 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
SIX1 antibody LS-C110311 Western blot of PANC1 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

SIX1 Antibody (C-Terminus, HRP) LS-C437424
Rabbit Polyclonal to Human SIX1
Pig, Human, Chimpanzee
WB
HRP Conjugated
100µl 0.65mg/ml  $385.00
SIX1 antibody LS-C110311 Western blot of PANC1 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
SIX1 antibody LS-C110311 Western blot of PANC1 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

SIX1 Antibody (C-Terminus, Biotin) LS-C437422
Rabbit Polyclonal to Human SIX1
Pig, Human, Chimpanzee
WB
Biotin Conjugated
100µl 1mg/ml  $385.00
SIX1 antibody LS-C110311 Western blot of PANC1 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
SIX1 antibody LS-C110311 Western blot of PANC1 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

SIX1 Antibody (C-Terminus, FITC) LS-C437423
Rabbit Polyclonal to Human SIX1
Pig, Human, Chimpanzee
WB
FITC Conjugated
100µl 1mg/ml  $385.00
SIX1 antibody LS-C110311 Western blot of PANC1 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
SIX1 antibody LS-C110311 Western blot of PANC1 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

SIX1 Antibody LS-C410185
Rabbit Polyclonal (IgG) to Human SIX1
Mouse, Human
WB
Unconjugated
100µl  $295.00; 200µl  $385.00
Western blot analysis of extracts of HepG2 cells.
Western blot analysis of extracts of HepG2 cells.

SIX1 Antibody (aa245-282) LS-C408101
Rabbit Polyclonal to Human SIX1
Human
WB
Unconjugated
100µg  $325.00
Western blot analysis of SIX1 expression in MCF-7 whole cell lysates (lane 1) and 22RV1 whole cell lysates (lane 2). SIX1 at 37 kD was detected using rabbit anti- SIX1 Antigen Affinity purified polyclonal antibody at 0.5 ug/mL. The blot was developed using chemiluminescence (ECL) method.
Western blot analysis of SIX1 expression in MCF-7 whole cell lysates (lane 1) and 22RV1 whole cell lysates (lane 2). SIX1 at 37 kD was detected using rabbit anti- SIX1 Antigen Affinity purified polyclonal antibody at 0.5 ug/mL. The blot was developed using chemiluminescence (ECL) method.

SIX1 Antibody LS-C490560
Rabbit Polyclonal (IgG) to Human SIX1
Human
WB
Unconjugated
100µg  $365.00
Western blot Image
Western blot Image

SIX1 Antibody LS-C747197
Rabbit Polyclonal (IgG) to Human SIX1
Mouse, Rat, Human
WB
Unconjugated
50µl  $235.00
Western blot analysis of extracts of various cell lines, using SIX1 antibody at 1:3000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 20s.
Western blot analysis of extracts of various cell lines, using SIX1 antibody at 1:3000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 20s.
Showing 1-22 of 22 results

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PLEASE NOTE

For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).


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